ALPHA 2,6 SIALYLTRANSFERASE ELISA KIT; 200pg/ml sensitivity (96T, Human, mouse or rat, Part No. 27762: kw. alpha 26 syalyltransferase, a26st, a26 sialyltransferase)

Alpha 2,6 - Sialyltransferase ELISA Kit

Human, mouse and rat reactivity

 

Sensitive colorimetric solid phase ELISA kit to a2,6-Sialyltransferase with assay range of 1.09 - 70 ng/mL.  Selective to alpha2,6-Sialyltransferase in human, mouse, and rat serum, EDTA plasma, and cell culture supernatant.   

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Specifications:

1. Complete 96T ELISA kit in sandwich format.  Colorimetric detection at 450 nm.
   
2. ELISA plate is coated with an affinity purified monoclonal antibody that recognizes alpha 2,6-Sialyltransferase.
3. The kit detects alpha2,6-Sialyltransferase in human, mouse, and rat serum, EDTA plasma, and cell culture supernatant.
4. EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
5. Assay range:  1.09 - 70 ng/ml,  sensitivity: 0.2ng/ml
   
6. Imported from Japan

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Background on Alpha 2,6 Sialyltransferase

alpha2,6-sialyltransferase is a protein that transfers sialyc acid onto carbohydrate chains.  It is cleaved by Beta-secretase (BACE1), a protein that also cleaves Amyloid precursor protein, APP, to form the Beta-Amyloid (aBeta) plaques in neurons characterizing the initial lesion of Alzheimer's disease.  In rats, the cleaved alpha 2,6-sialyltransferase is in the E41 Form. 

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1. Equilibrate plate to RT
   
2. Add sample to plate, incubate overnight
   
3. Add detection antibody, incubate 1 hr
   
4. Add color reagents, 30 min
   
5. Add stop solution.  Measure OD at 450nm

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References:

1. Kitazume-Kawaguchi S, Dohmae N, Takio K, Tsuji S, Colley KJ. The  relationship between ST6Gal I Golgi retention and its cleavage-secretion. : Glycobiology. 1999 Dec;9(12):1397-406.
2. Kitazume S,  Tachida Y,  Oka R, Shirotani K,  Saido TC,   Hashimoto Y. Alzheimer's beta-secretase, beta-site amyloid precursor protein-cleaving enzyme, is responsible for cleavage secretion of a Golgi-resident sialyltransferase. : Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13554-9.
3. Kitazume S, Tachida Y, Oka R, Kotani N, Ogawa K,  Suzuki M, Dohmae N, Takio N, Saido TC, Hashimoto Y. Characterization of alpha 2,6-Sialyltransferase Cleavage by Alzheimer's beta -Secretase (BACE1). : J  Biol Chem. 2003 Apr 25;278(17):14865-71.

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