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ECL Chemiluminescence Kit | FIVEphoton Biochemicals | PCD-250, PCD-500 | High Sensitivity

ECL Chemiluminescence Kit | FIVEphoton Biochemicals | PCD-250, PCD-500 | High Sensitivity
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Price: $179.95
Availability: In Stock
Model: PCD-500
Manufacturer: Fivephoton Biochemicals

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ECL Photon Chemiluminescence Western Blotting  Detection Kits (PCD-250TM and PCD-500TM )

Kits provide 250 ml of ECL substrate suitable for 2500 cm2 membrane area (PCD-250TM) or 500 ml of ECL substrate, suitable for 5000 cm2 membrane area (PCD-500TM) . 

Highlights
  •   Strong signal ECL Western blotting protein detection method
  •   Sensitive picogram detection
  •   Applicable to PVDF or nitrocellulose membranes.  Develop on film or CCD camera
  •   Effective tool for publication quality Western Blot figures

View Protocol

Available ECL Kit Options

 Part No
 Membrane Area Coverage
 Price
 PCD-250
 2500 cm2  
$179.95
 PCD-500
 5000 cm2   $214.95
+Select kit in Available Options menu


ECL Kit Contents
PCD-250TM:   Cost, $179.95
Suitable for 2500 cm2 membrane area.
  1. ECL substrate:  250 ml (Solution A)
  2. Activator:  1 ml (Solution B)

PCD-500TM:   Cost, $214.95
Suitable for 5000 cm2 membrane area.
  1. ECL substrate:  500 ml (Solution A)        
  2. Activator:  2 ml (Solution B)

The Photon ECL Chemiluminescence Western Blotting Detection Kit (PCD) contains reagents to rapidly and reliably detect proteins in Western blots to picogram protein amounts. The unique feature of this ECL reagent kit is its high signal to noise luminescence, providing outstanding selectivity and robust signal.

 


ECL Chemiluminescence Western Blot Detection (PCD) Kit Overview
The horseradish peroxidase (HRP)-catalyzed enhanced chemiluminescence method is commonly known as the ECL detection method for Western blots.  Proteins in Western blots are recognized by specific primary antibodies, which are in turn detected by a secondary antibodies coupled to horse-radish peroxidase (HRP).  In the ECL reaction, HRP catalyzes luminol oxidation in the presence of peroxidase and enhancer.  The resulting luminescence that corresponds to protein bands in Western blots is detected on film or by a CCD camera.

 
ECL Chemiluminescence Detection Kit:  Protocol Synopsis
 
 
To detect protein bands transferred to PVDF or nitrocellulose membrane, premix solution A, which contains the peroxidase substrate, luminol and enhancer, with the peroxide solution B.  Incubate the membrane with the premixed solution for one minute.  Bright luminescence will be emitted that can be captured on film or a CCD camera.

Figure 1: PCD is more sensitive than GE ECL Substrate

Legend: PCDTM ECL Kit sensitivity.  Recombinant human IL-10 (hIL-10) was diluted (25-0.05 ng)  and electrophoresis was performed. The gels were transferred to PVDF membranes, blocked and incubated with a 0.5 µg/ml dilution of rabbit anti-IL-10 antibody.  After washing, the membranes were incubated with 1:5000 dilutions of HRP-conjugated goat anti-donkey antibody.  The membranes were washed again and then incubated with chemiluminescent substrate.  Blots were exposed to film for 30 second and 2 minute exposures.  The FIVEphoton Biochemicals ECL reagent kit detects picogram amounts of protein.

Additional examples of Western blots developed with the FIVEphoton Biochemicals ECL Kit

Product Citations
  1. Xie, R., Dong, X., Wong, C., Vallon, V., Tang, B., Sun, J., ... & Dong, H. (2014). Molecular Mechanisms of Calcium-sensing Receptor-mediated Calcium Signaling in the Modulation of Epithelial Ion Transport and Bicarbonate Secretion. Journal of Biological Chemistry, 289(50), 34642-34653.
  2. Yu, H. L., Gao, L. F., Ma, W. W., Xie, F., Bi, Y. X., Yuan, L. H., ... & Xiao, R. (2013). The effects of phytosterol supplementation on serum LDL-C levels and learning ability in mice fed a high-fat, high-energy diet from gestation onward. International journal of food sciences and nutrition, 64(6), 724-729.

Safety:  Harmful.  Avoid ingestion, eye and skin contact.
Storage:  4oC.
Shipping Options:  2 day-AM continental US Fedex shipping ($22; flat rate) or overnight Fedex domestic delivery ($43;  flat rate).  International delivery is also available;  please inquire for international shipping rate to your location.


 

Category Download Link
Protocol Manual click here
MSDS click here



 

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