AMYLOID PROTIEN BINDING PROTEIN 2 (APPBP2, human, mouse, rat) ELISA KIT (h, r, m, APPBP2-ELISA)

Amyloid Protein Binding Protein 2 (APPBP2) ELISA Kit

Separate kits available for human, mouse and rat

Introduction

The APPBP2 protein interacts with microtubules and is functionally associated with beta-amyloid precursor protein transport. The beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease.

Product name	Appbp2 (Amyloid protein-binding protein 2) ELISA Kit
Catalogue Nos.	h,r,m, APPBP2-ELISA
Alias	APPBP2 ELISA
Detection method	Sandwich ELISA, Double Antibody
Size	96T
Range	0.313-20ng/ml Human;  0.156-10ng/ml Mouse and Rat
Sensitivity	< 0.188ng/ml human;  < 0.094ng/ml mouse/rat
Standard curve	Human APPBP2 ELISA Kit
Storage	4 ℃ for 6 months
Recovery	Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples. Matrix Recovery range(%) Average(%) serum(n=5) 86-105 95 EDTA plasma(n=5) 89-103 96 heparin plasma(n=5) 85-105 98
Linearity	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 89-98% 87-102% 94-103% 86-99% EDTA plasma(n=5) 82-98% 83-101% 86-96% 88-95% heparin plasma(n=5) 80-100% 80-99% 87-97% 82-98%
CV(%)	Intra-Assay: CV<8% Inter-Assay: CV<10%
Note	For Research Use Only

1. Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C
   
2. Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample
   
3. Standard dilution buffer 10ml/20ml 4°C
   
4. Biotin-detection antibody (Concentrated) 60ul/120ul 4°C
   
5. Antibody dilution buffer 5ml/10ml 4°C
   
6. HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive)
   
7. SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive)
   
8. Stop solution 5ml/10ml 4°C
   
9. Wash buffer (25X) 15ml/30ml 4°C
   

Protocol Summary