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AMH, Human Anti-Mullerian Hormone 0.2-60ng/ml Assay Range ELISA Kit 96T (hAMH-ELISA)

AMH, Human Anti-Mullerian Hormone 0.2-60ng/ml Assay Range ELISA Kit 96T (hAMH-ELISA)
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Price: $640.00
Availability: In Stock
Model: hAMH-ELISA

DetailsManuals & Downloads
AMH, Anti-Mullerian Hormone (AMH; Part hAMH-ELISA) ELISA Kits 
Sandwich ELISA format (96T)
 
Highlights
  • Assay ranges 0.2 ng/ml to 60 ng/ml.
  • Sandwich ELISA format providing convenience and high sensitivity. 
  • Strip plate format allowing separate use and storage of sections of the ELISA plate.
  • Rapid protocol:  Only one incubation with sample to antibodies.  Total protocol time approximately 1.5 hrs.
  • Colormetric detection at 450nm using a standard absorbance plate reader.

Price (USD)
Number of Kits
Number of Assays
$640
1
96T



 
Specifications
Species
 Part
Standard Peptide
Assay Range
Human
 hAMH-ELISA
16 ng/ml
0.01 ng/ml - 15 ng/ml
                               




Anti-Mullerian Hormone (AMH) is one of the primary sexual differentiation factors in fetuses.  It causes the Mullerian ducts to degenerate so that the testes can form; otherwise the ovaries would form instead.  Also known as Mullerian Inhibiting Factor (MIF), it is found in both male and female fetuses and individuals throughout their lives, though it is more strongly expressed in male sertoli cells where it regulates other sex hormones, falling in males during puberty and rising in females in the same period.    

AMH  levels in the bloodstream can be used to guage the amount of eggs a woman has in her ovarian reserve, a useful fertility test.  Anti-Mullerian Hormone in the uterus prevents the excessive formation of primary follicles. 

In embryonic mice, AMH found in the brain may be related to the development of sexually dimorphic behavior patterns in individual mice. 


Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.  

   Sandwich ELISA Methods to Anti-Mullerian Hormone (AMH)                                                                                                   

ELISA Assay Steps
  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.

References on anti-mullerian
  1. JOSSO, NATHALIE. "ANTI‐MÜLLERIAN HORMONE." Clinical endocrinology 25.3 (1986): 331-345.
  2. Weenen C, Laven JS, Von Bergh AR, Cranfield M, Groome NP, Visser JA, Kramer P, Fauser BC, Themmen AP (Feb 2004). "Anti-Müllerian hormone expression pattern in the human ovary: potential implications for initial and cyclic follicle recruitment". Molecular Human Reproduction 10 (2): 77–83. doi:10.1093/molehr/gah015. PMID 14742691
  3. Trbovich AM, Martinelle N, O'Neill FH, Pearson EJ, Donahoe PK, Sluss PM, Teixeira J (Oct 2004). "Steroidogenic activities in MA-10 Leydig cells are differentially altered by cAMP and Müllerian inhibiting substance". The Journal of Steroid Biochemistry and Molecular Biology 92 (3): 199–208. doi:10.1016/j.jsbmb.2004.07.002. PMID 15555913.
  4. Wang PY, Protheroe A, Clarkson AN, Imhoff F, Koishi K, McLennan IS (Apr 2009). "Müllerian inhibiting substance contributes to sex-linked biases in the brain and behavior". Proceedings of the National Academy of Sciences of the United States of America 106 (17): 7203–8. doi:10.1073/pnas.0902253106. PMC 2678437. PMID 19359476.
  5. Usta, T., & Oral, E. (2012). Is the measurement of anti-Müllerian hormone essential?. Current Opinion in Obstetrics and Gynecology, 24(3), 151-157.
Safety, Storage, Shipping, AMH ELISA Kit
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC.  Expires 6 months after manufacture.
Shipping.  Lead time estimate:  2 weeks.  Overnight domestic delivery recommended.  Also available for international delivery.  Please inquire for rates.


 

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Protocol Manual click here
MSDS click here