COMT, Catechol-O-Methyltransferase, 50pg-3ng/ml Assay Range For Human, ELISA Kit (Part h, r, m, p COMT-ELISA, Available for human, mouse, rat, porchine )
Specifications of COMT, Catechol-O-Methyltransferase ELISA Kits
Format 1.
Species
Standard
Assay Range
Price
Human
80 ng/ml
0.2 ng/ml - 60 ng/ml
$590
Mouse
80 ng/ml
0.2 ng/ml - 60 ng/ml
$620
Rat
80 ng/ml
0.2 ng/ml - 60 ng/ml
$620
Porcine
80 ng/ml
0.2 ng/ml - 60 ng/ml
$640
Format 2.
Species
Standard
Assay Range
Price
Human
4.5 ng/ml
50 pg/ml - 3000 pg/ml
$590
Rat
2.7 ng/ml
20 pg/ml - 1200 pg/ml
$620
Mouse
2.7 ng/ml
30 pg/ml - 1800 pg/ml
$620
Porcine
80 ng/ml
0.2 ng/ml - 60 ng/ml
$640
Use Available Options menu to select kit and species.
Background: Catechol-O-Methyltransferase (COMT) is responsible for degrading catecholamines, including neurotransmitters like dopamine, norepinephrin, and epinephrin by adding a methyl group to its substrate's catecholamine group. It is mostly found in neurons throughout the central nervous system and in the liver. Mutations of COMT are associated with psychological disorders such as scizophrenia and bipolar disorder.
Methylation reaction catalyzed by Catechol-O-Methyltransferase:
Methods Overview
Sandwich ELISA Format 1: The ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with provided biotin-linked detection antibody and streptavidin-HRP solutions, which are then added to the plate and incubated for 60min. The plate is washed and color reagents are added, resulting in a reaction with HRP. A stop solution is applied and the resulting yellow solution is measured at 450nm.
Sandwich ELISA Format 2: The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. This assay requires approximately 2.5 hrs to complete at RT and approximately 1.25 hrs with 37oC incubations.
*(Use the protocol shipped with the kit for your experiment).
Image: Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40). PDB: DOI:10.2210/pdb2g47/pdb Representative Sandwich ELISA Methods
Steps of Sandwich ELISA Format 1
Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC. Wash 5 times.
Apply chromogens, incubate, then stop reaction.
Steps of Sandwich ELISA Format 2
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
Protocols
Format 1
Format 2
References on Catechol-O-Methyltransferase:
Lachman, H. M., Papolos, D. F., Saito, T., Yu, Y. M., Szumlanski, C. L., & Weinshilboum, R. M. (1996). Human catechol-O-methyltransferase pharmacogenetics: description of a functional polymorphism and its potential application to neuropsychiatric disorders. Pharmacogenetics and Genomics, 6(3), 243-250.
Männistö, P. T., & Kaakkola, S. (1999). Catechol-O-methyltransferase (COMT): biochemistry, molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors. Pharmacological reviews, 51(4), 593-628.
BALL, P., KNUPPEN, R., HAUPT, M., & BREUER, H. (1972). Interactions Between Estrogens and Catechol Amines III. Studies on the Methylation of Catechol Estrogens, Catechol Amines and other Catechols by the Catechol-O-Methyltransferase 1 of Human Liver. The Journal of Clinical Endocrinology & Metabolism, 34(4), 736-746.
Akil, M., Kolachana, B. S., Rothmond, D. A., Hyde, T. M., Weinberger, D. R., & Kleinman, J. E. (2003). Catechol-O-methyltransferase genotype and dopamine regulation in the human brain. The Journal of neuroscience, 23(6), 2008-2013.
For research only. Not for diagnostics of disease in humans or animals.
Storage: 4oC, 6 months. Longer term storage of sections of the strip plate and standards at -20oC. Seal and store 96-well ELISA plate in a zip locks bag after opening.
Shipping. Overnight domestic delivery recommended (Flat rate Fedex cost for continental US: $45). Also available for international delivery; please inquire for shipping cost.
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