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Human Neprilysin (NEP) ELISA Kit ( Part NEP-ELISA Biotin) kw: neurosin

Human Neprilysin (NEP) ELISA Kit ( Part NEP-ELISA Biotin) kw: neurosin
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Price: $655.00
Availability: In Stock
Model: NEP-ELISA Biotin

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Human Neprilysin (NEP) ELISA Kit

Dynamic Assay Range: 5pg/ml - 1000pg/ml.  Sensitivity: 3.8pg/ml.
Lead Time:  Approximately 2 weeks
 
Fully guaranteed to meet specifications

Specifications
  • Fully pre-configured ELISA kit in sandwich assay format.
  • Dynamic Assay Range: 5pg/ml - 1000pg/ml.  Sensitivity: 3.8pg/ml.
  • Rapid ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate.
  • Available in 96T format.

Number of Assays
 Cost
96T
 $655
2 x 96T
 $1200

 

Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a provided biotin-linked detection antibody and provided streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
  Protocol
 
   Sandwich ELISA Methods to NEP                                                      

ELISA Assay Steps
  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.


 
 
Gerneral References on NEP
 
1. Papandreou CN, Nanus DM (January 2010). "Is methylation the key to CD10 loss?". J. Pediatr. Hematol. Oncol. 32 (1): 2–3.
2. Wang DS, Iwata N, Hama E, Saido TC, Dickson DW (October 2003). "Oxidized neprilysin in aging and Alzheimer's disease brains". Biochem. Biophys. Res. Commun.310 (1): 236–41.
3. Oefner C, Roques BP, Fournie-Zaluski MC, Dale GE (February 2004). "Structural analysis of neprilysin with various specific and potent inhibitors". Acta Crystallogr. D Biol. Crystallogr. 60 (Pt 2): 392–6.
 
 
For research only.  Not for diagnostics.
 
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4°C. 6 months.
Shipping.  Overnight domestic delivery recommended with ice packs.  Also avaialble for international delivery; please inquire for shipping cost. 


 

Category Download Link
Protocol Manual click here
MSDS click here



 

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