HUMAN AMYLOID-BETA 1-42 12.5-800pg/ML Assay Range, ELISA KIT, IBL 27711: kw. AB42, beta-amyloid 1-42, amyloid beta

Human Amyloid-Beta 1-42 ELISA Kit (96T)

Sensitive and selective colorimetric ELISA kit to ABeta (1-42) with assay range 12.5-800 pg/ml. Highly selective to amyloid-beta 1-42 with minimal cross reactivity to amyloid-beta 1-40 (see data below). The affinity purified polyclonal capture antibody is selective to the C-terminus at amino acid position 42 and the affinity purified monoclonal-HRP detection antibody recognizes an epitope near amino acid position 20 in Abeta 1-42.

A related and similarly configured ELISA kit to Abeta 1-40 is also available and can be purchased as a pair with this kit (see pull down menu).

Specifications:

12.5 - 800 pg/ml assay range, 4.03 pg/ml sensitivity.
Complete ELISA kit in sandwich ELISA format. Colorimetric detection at 450 nm. 96T
ELISA plate is coated with an affinity purified polyclonal capture antibody that recognizes the C-terminus near position 42 of amyloid-beta 1-42. The detection antibody is a monoclonal that recognizes an epitope near to amino acid position 20.
The kit detects amyloid-beta 1-42 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
Incubation times: Samples with plate; overnight. Detection antibody: 1 hr

Protocol Overview

Protocol

Epitope Positions

Background on Amyloid-Beta (1-42)

Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. The prevailing isoforms of Amyloid-Beta are Aβ^₄₀ and Aβ^₄₂. Aβ^₄₀ is the most widespread amyloid-Beta (Aβ) isoform, while Aβ^₄₂ is more fibrillogenic and is the principal Abeta peptide that may be associated with Alzheimer’s disease.

We now also carry a line of ELISA kits with targets that appear to be associated with infection and inflammatory responses that may be associated with dimentia diseases. For example, alpha-1-proteinase inhibitor (α1-PI), C-reactive protein (CRP), and leukocyte elastase (LE). To locate this group of ELISA kits, enter the target name in the search bar.

Methods Overview

Equilibrate plate to RT
Add sample to plate, incubate overnight
Add detection antibody, incubate 1 hr
Add color reagents, 30 min
Add stop solution. Measure OD at 450nm

Amyloid-beta 1-42 ELISA Kit Validation Data (also see protocol manual)

References

Selkoe DJ. Normal and abnormal biology of theβ-Amyloid precursor protein. Annu. Rev. Neurosci. 17: 489-517, 1994.
Wang R, Sweeney D, Gandy SE, and Sisodia SS. The profile of soluble amyloidβ protein in cultured cell media. J. Biol. Chem. 271: 31894-31902, 1996.
Saido T.C., Iwatsubo T., Mann D.M.A., Shimada H., Ihara Y., and Kawashima S. Dominant and differential deposition of distinct -amyloid peptide species, AβN3(pE), in senile plaques. Neuron 14, 457-466, 1995.
Horikoshi Y., Mori T., Maeda M., Kinoshita N., Sato K., Yamaguchi H., Aβ N-terminal-end specific antibody reduced β-amyloid in Alzheimer-model mice. Biochem. Biophys. Res. Commun. 325: 384-387, 2004.

Storage: 4^oC, 1 year

Safety: Stop solution contains acid. Avoid contact, wear eye protection.

Shipping: Lead time estimate: 2 weeks. Overnight delivery to continental United States addresses with styrofoam box and ice packs. International delivery is also available: Please inquire for cost

kw: AMYLOID BETA, BETA AMYLOID, ABETA, AB, 1-42, 42, ELISAS KIT

Category	Download Link
Protocol Overview	click here
Protocol Manual	click here
MSDS	click here

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ADAM10 ELISA KIT, 50-1500pg/ml Assay Range, (A Disintegrin And Metalloprotease 10; Part No. ADAM10-ELISA (96T); kits available for human, rabbit, rat and mouse ADAM10-ELISA (96T) $810.00	ALPHA-SYNUCLEIN, SNCA, ELISA KIT; 125pg/ml sensitivity (a-synuclein, alpha synuclein elisa; Part h,m,r hsnca, 96T) kw SNCA, alpha synuclein, synuclein, a-synuclein) hSNCA $645.00	Human Amyloid-Beta 1-40 (Full length Abeta 1-40) ELISA Kit, Part No. 27714 IBL: kw. AB40N, beta-amyloid 1-40 N, amyloid beta 27714 $599.00	HUMAN AMYLOID-BETA 1-38 (FL) ELISA KIT (27717; kw. AB38, beta-amyloid 1-38, amyloid beta) 27717 $840.00
HUMAN AMYLOID-BETA 1-42N ELISA KIT, 6.25- 400 pg/mL Assay Range, IBL 27712: kw. AB42, beta-amyloid 1-42N, amyloid beta 27712 $639.00	Human Amyloid-Beta Oligomers (82E1-specific) ELISA Kit, Part No. 27725 IBL: kw. beta-amyloid oligomers, amyloid beta oligomers IBL 27725 $867.00	HUMAN AMYLOID BETA TOTAL 1-x ELISA KIT (27729: kw. AB1-x, beta-amyloid 1-x, ABETA IBL 27729 $679.00	HUMAN AMYLOID-BETA 1-42 12.5-800pg/ML Assay Range, ELISA KIT, IBL 27711: kw. AB42, beta-amyloid 1-42, amyloid beta IBL 27711 $720.00
HUMAN AMYLOID-BETA 1-42 ELISA KIT (27719: kw. AB42, beta-amyloid 1-42, amyloid beta) 27719 $840.00	HUMAN sAPP BETA WT ELISA KIT (No. 27732: kw. sAPPbeta, soluble amyloid precursor, appb) 27732 $675.00	HUMAN sAPP ALPHA ELISA KIT, sAPPAlpha, SAPPA, Soluble Amyloid Precursor Protein Alpha, IBL 27734: kw. sAPP-alpha, sAPPalpha, soluble amyloid precursor protein 27734 $670.00	HUMAN AMYLOID-BETA 1-40 ELISA KIT, (27713: kw. AB40, beta-amyloid 1-40, amyloid beta 40) 27713 $660.00
Human BACE1 ELISA Kit (Human Beta-site APP Cleaving Enzyme 1, Part No. 27752 IBL: kw. BACE1 27752 $660.00	ADAM10 ELISA KIT (A Disintegrin And Metalloprotease,100 pg-20ng/ml Assay Range: human, mouse or rat; Part hADAM10-ELISA Biotin kw: ADAM10, neurosin, ADAM 10, Desintegrin, Metalloprotease) hADAM10-ELISA Biotin $645.00	Human Amyloid-Beta N3pE-40 ELISA Kit, 2.34- 150 pg/mL Assay Range, Part No. 27418 IBL: kw. ABN3pE-40, beta-amyloid N3pE-40, amyoid beta N3pE-40 IBL 27418 $645.00	HUMAN AMYLOID-BETA 1-43 (FL) 2.34- 150 pg/mL Assay Range ELISA KIT, , IBL-27710: kw. AB43, beta-amyloid 1-43, amyloid beta IBL 27710 $840.00
HUMAN AMYLOID BETA 1-40 ELISA KIT 1pg/ml SENSITIVITY (27718; ABETA 1-40, AB40) 27718 $570.00	Human APLP2 (Amyloid-Like Protein 2; Part APLP2-ELISA APLP2-ELISA $585.00	AMYLOID PROTIEN BINDING PROTEIN 2 (APPBP2, human, mouse, rat) ELISA KIT (h, r, m, APPBP2-ELISA) h,r,m,APPBP2-ELISA $655.00	Human Soluble α-Klotho ELISA Kit (alpha-klotho, alpha klotho, 96T; 27998) 27998 $773.00
Human Beta Klotho (KLB) ELISA Kit (96T, beta-klotho, hKLB-ELISA) hKLB-ELISA $725.00

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