HUMAN AMYLOID-BETA 1-38 (FL) ELISA KIT (27717; kw. AB38, beta-amyloid 1-38, amyloid beta)

Amyloid-Beta 1-38 (FL) ELISA Kit (Human Specificity)  96T

Sensitive and selective colorimetric ELISA kit to ABeta (1-38);  assay range 9.38-600 pg/ml.  Highly selective to amyloid-beta 1-38 with minimal cross reactivity to other processed forms of amyloid-beta (see data below).  The affinity purified monoclonal capture antibody reacts with the N-terminus and the affinity purified monoclonal-HRP detection antibody reacts with the C-terminus of ABeta 1-38.  Insignificant cross-reactivity with Abeta1-40 and Abeta 1-42.   Highly validated 96T Sandwich ELISA kit.

Related kits to Abeta 1-40 and Abeta 1-42 are also available.

1. Complete 96T ELISA kit in sandwich format.  Colorimetric detection at 450 nm.
   
2. ELISA plate is coated with an affinity purified monoclonal antibody that recognizes the N-terminus of amyloid-beta 1-38:  The detection antibody is also a monoclonal antibody, and reacts with the C-terminus of Abeta1-38.
   
3. The kit detects amyloid-beta 1-38 in cerebrospinal fluids and cell culture media.
4. EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
5. Incubation times:  Overnight sample incubation.  Detection antibody:  1 hr
   
6. Assay range:  9.38 - 600 pg/ml; sensitivity:  1.28 pg/ml

  Protocol Overview

  Protocol

  Epitope Positions

Background on Amyloid-Beta 1-38 (FL)

Amyloid-beta (Abeta) peptides of various lenghts are processed from amyloid precursor protein (APP) cleavage by secretases.  Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues.  The prevailing isoforms of Amyloid-Beta are Aβ₄₀ and Aβ₄₂.  Aβ₄₀ is the most widespread amyloid-Beta (Aβ) isoform, while Aβ₄₂ is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease.  Antibodies used in this ELISA kit are selective to Aβ₃₈ , with insignificant cross-reactivity to Aβ forms that extend C-terminus to the 38 amino acid position"  i.e  there is insignificant reactivity with Aβ_40, &  Aβ_42.

1. Equilibrate plate to RT
   
2. Add sample to plate, incubate overnight
   
3. Add detection antibody, incubate 1 hr
   
4. Add color reagents, 30 min
   
5. Add stop solution.  Measure OD at 450nm