Rabbit Amyloid-Beta ELISA Kits (1-40). Part No. rAB1-40ELISA, kw. beta amyloid, ABeta, AB

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Background on Targets of the Amyloid ELISA Kits 

Amyloid-Beta (Aβ) is generated by sequential cleavage of the amyloid precursor protein (APP) by beta and gamma secretases.  The γ secretase, which produces the C-terminal end of the Aβ peptide, cleaves within the transmembrane region of APP and generates amyloid-Beta (Aβ) isoforms of 36-43 amino acid residues in length.  The predominant isoforms of amyloid-Beta are Aβ₄₀ and Aβ₄₂.  The shorter amyloid-Beta isoform is typically produced by cleavage in the endoplasmic reticulum, while the longer isoform is processed by cleavage in the trans-Golgi network.  Aβ₄₀ is the most common amyloid-Beta (Aβ) isoform, but Aβ₄₂ is more fibrillogenic and is thus the predominant form associated Alzheimer’s disease.  Antibodies employed in this Amyloid-Beta ELISA kit are selective for the Aβ₄₀ isoform of amyloid-beta, with insignificant cross-reactivity to the Aβ₄₂ isoform.

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Representative Sandwich ELISA Methods

Steps of Sandwich ELISA
1.  Equilibrate plate to ambient temperature

2.  Add sample, incubate 30 min - 1hr, wash 

3.  Add HRP-Antibody, incubate 30 min - 1 hr, wash

4.  Add the color reagent, incubate 15 min

5.  Add the stop solution

6.  Colorimetric detection, 450 nm

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Sandwich ELISA Method Overview

The included 96-well ELISA micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen.  Upon addition of samples to the wells, the antigen binds to the antibody and becomes indirectly linked to the solid support.  After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period.  Unbound antibody is washed, and color reagents are added, which upon conversion by HRP, become blue colored.  A stop solution is then added to block further reaction between HRP and the colorimetric substrates, causing the solution to become yellow.  An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm.

These ELISA kits provide a standard peptide solution with known concentration to calibrate absorbance readings to concentration.

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Safety, Storage, Shipping

Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.

Storage:  4^oC, 6 months.  Strip plate sections, standard and other solutions can be stored at -20^oC longer term.

Shipping.  Overnight domestic delivery recommended (cost $52).  International shipping also available:  Inquire for shipping rate.