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InformationsAPP BETA (wt) ELISA KIT, HIGH SENSITIVITY (sAPPbeta; Kits to Human, Rat, Mouse, Rabbit: Part No sAPPbeta-ELISA)
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Soluble Amyloid Precursor Protein Beta (wt) ELISA Kits (sAPPß wt). Separate Kits For Human, Rat, Mouse and Rabbit Email customersupport@fivephoton.com for availability
Use the Available Options menu to select the ELISA kit by species.
Lead time: Approximately 2 weeks.
Fully guaranteed to meet specifications.
Highlights
sAPPß (wt) ELISA Kit Availability And Specifications
Use Available Options menu to select the species specificity of the sAPPbeta ELISA Kits.
Related Kits
* An alternate sAPPbeta ELISA kit with assay range of 0.78-50ng/ml is available that uses a monoclonal capture antibody and a
polyclonal detection antibody (Cost: $599). click here
* An alternate total sAPP ELISA kit with assay range of 0.39-25ng/ml is available that uses a monoclonal capture antibody and a polyclonal
detection antibody (Cost: $599). click here
Background
sAPPbeta is the extracellular soluble fragment of amyloid precursor protein (APP) cleaved by beta-secretase into a 654 amino acid peptide. Cleavage of APP by beta-secretase in the extracellular domain is the first cleavage processing step generating amyloid-beta peptide. Membrane embedded gama-secretase subsequently cleaves APP within the transmembrane domain and generates amyloid-beta peptide of variable length.1 This sAPP beta ELISA detects the sAPP beta peptide in cell culture medium and biological fluids, including CSF.
1. Augutis K, et. al. 2012. Cerebrospinal fluid biomarkers of β-amyloid metabolism in multiple sclerosis. Mult Scler. 2012 Oct 15. [Epub ahead of print]
Methods Overview:
The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal capture antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. The assay requires approximately 1.5 hrs to complete at 37oC, which includes incubation with antibodies, washes and color reaction.
This ELISA assay kit provides a standard peptide solution with known concentration to directly calibrate absorbance readings to concentration.
Representative Sandwich ELISA Methods 
Steps of Representative Sandwich ELISA
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
Protocol
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC, 6 months. Strip plate sections, standard and other solutions can be stored longer term at -20oC.
Shipping. Overnight domestic delivery recommended with styrofoam box/ice pack. Also available for international delivery; please inquire for shipping cost.
sAPPbeta, soluble amyloid precursor beta, ELISA, sAPPb, amyloid beta, amyloid precursor protein, alzheimer's
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