MOUSE TNF-alpha ELISA KIT (96T; mTNFalpha-ELISA)
Click to enlarge |
| Price: |
$625.00 |
| Availability: |
In Stock |
| Model: |
mTNFalpha-ELISA |
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Mouse TNF-a (Tumor Necrosis Factor - Alpha) ELISA Kit
 Protocol
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Product |
Mouse TNFα (Tumor Necrosis Factor Alpha) ELISA Kit
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Product No. |
mTNFalpha-ELISA
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Detection |
Sandwich ELISA, Double Antibody
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Wells |
96T
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Assay Range |
31.25-2000pg/ml
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Sensitivity |
< 18.75pg/ml
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Standard curve |
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Storage |
4 ℃ for 6 months
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Recovery |
Matrices listed below were spiked with TNFα (Tumor Necrosis Factor Alpha) /TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFα (Tumor Necrosis Factor Alpha) /TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
94 |
|
EDTA plasma(n=5) |
86-103 |
97 |
|
heparin plasma(n=5) |
91-102 |
96 |
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Linearity |
The linearity of the kit was assayed by testing samples spiked with TNFα (Tumor Necrosis Factor Alpha) /TNF-α/DIF/TNF-alpha/TNFA/TNFSF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum (n=5) |
93-102% |
90-105% |
85-100% |
87-102% |
|
EDTA plasma (n=5) |
86-101% |
82-99% |
82-93% |
89-101% |
|
heparin plasma( n=5) |
80-96% |
82-99% |
81-89% |
85-96% |
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CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10%
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Note |
For Research Use Only
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Storage: 4oC 6 months
Shipping: Overnight delivery with ice packs. International shipping is available for this item.
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