Transthyretin (TTR) ELISA Kit (h, m, r, TTR-ELISA)

Separate kits are available for human, mouse, rat.  96T

Fully guaranteed to meet specifications

Introduction:

TTR misfolding and aggregation is known to be associated with thee amyloid diseases senile systemic amyloidosis (SSA), familial amyloid polyneuropathy (FAP), and familial amyloid cardiomyopathy (FAC).  Transthyretin tetramer dissociation is known to be rate-limiting for amyloid fibril formation. However, the monomer also must partially denature in order for TTR to be mis-assembly competent, leading to a variety of aggregate structures, including amyloid fibrils.  TTR is also thought to have beneficial side effects, by binding to beta-amyloid protein, thereby preventing beta-amyloid's accumulation into the plaques associated with the early stages of Alzheimer's Disease.  

      Protocol Mouse

      Protocol Rat

Product name	Human TTR(Transthyretin) ELISA Kit
Catalogue No.	h, m, r TTR-ELISA
Alias	TTR (Transthyretin) ELISA Kit, PALB ELISA Kit, TBPA ELISA Kit, ATTR ELISA Kit, Prealbumin ELISA Kit, HsT2651 ELISA Kit, prealbumin, amyloidosis type I ELISA Kit, thyroxine-binding prealbumin ELISA Kit
Detection method	Sandwich ELISA, Double Antibody
Application	TTR ELISA Kit allows for the in vitro quantitative determination of TTR concentrations in plasma, tissue homogenates and other biological fluids.
Size	96T
Range	15.625-1000ng/ml
Sensitivity	< 9.375ng/ml
Standard curve	Human TTR(Transthyretin) ELISA Kit
Storage	4 ℃ for 6 months
Recovery	Matrices listed below were spiked with certain level of TTR and the recovery rates were calculated by comparing the measured value to the expected amount of TTR in samples. Matrix Recovery range (%) Average(%) serum(n=5) 87-105 97 EDTA plasma(n=5) 86-102 96 heparin plasma(n=5) 90-102 97
Linearity	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TTR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 92-105% 92-105% 86-105% 85-104% EDTA plasma(n=5) 84-92% 84-97% 86-98% 84-101% heparin plasma(n=5) 81-89% 81-94% 85-91% 80-92%
CV(%)	Intra-Assay: CV<8% Inter-Assay: CV<10%

1. Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C
   
2. Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample
   
3. Standard dilution buffer 10ml/20ml 4°C
   
4. Biotin-detection antibody (Concentrated) 60ul/120ul 4°C
   
5. Antibody dilution buffer 5ml/10ml 4°C
   
6. HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive)
   
7. SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive)
   
8. Stop solution 5ml/10ml 4°C
   
9. Wash buffer (25X) 15ml/30ml 4°C
   

Protocol Summary