SERUM AMYLOID A (SAA) ELISA KIT (Human, Mouse, Rat; h, m, r, SAA-ELISA)

Introduction Serum amyloid A (SAA) proteins are a family of apolipoproteins associated with high-density lipoprotein (HDL) in plasma. Different isoforms of SAA are expressed constitutively (constitutive SAAs) at different levels or in response to inflammatory stimuli (acute phase SAAs). These proteins are produced predominantly by the liver. The conservation of these proteins through out invertebrates and vertebrates suggests that SAAs play a highly essential role in all animals. Protocol Human       Protocol Mouse       Protocol Rat

Product name	Serum Amyloid A ELISA Kit (Separate kits for human, mouse and rat)
Catalogue No.	h, m, r, SAA-ELISA
Alias	SAA (Serum Amyloid A) ELISA Kit
Detection method	Sandwich ELISA, Double Antibody
Application	SAA ELISA Kits allow for the in vitro quantitative determination of SAA concentrations in plasma, tissue homogenates and other biological fluids.  For research use only.
Size	96T
Ranges	Human:  1.25-80ng/ml;    Mouse:  1.563-100ng/ml;  Rat:  0.156-10ug/ml
Sensitivity	Human:  0.75ng/ml;          Mouse:  0.938ng/ml;        Rat:  .094ug/ml
Standard Curve (Human)	Human SAA(Serum Amyloid A) ELISA Kit
Storage	4 ℃ for 6 months
Recovery	Matrices listed below were spiked with certain level of SAA and the recovery rates were calculated by comparing the measured value to the expected amount of SAA in samples.     Matrix Recovery range (%) Average(%) serum (n=5) 91-104 98 EDTA plasma (n=5) 91-105 98 heparin plasma (n=5) 87-99 92
Linearity	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SAA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.       Sample 1:2 1:4 1:8 1:16 serum(n=5) 85-105% 85-104% 85-105% 85-103% EDTA plasma(n=5) 83-99% 82-97% 85-98% 82-97% heparin plasma(n=5) 83-94% 85-100% 90-99% 81-94%
CV(%)	Intra-Assay: CV<8% Inter-Assay: CV<10%

1. Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C
   
2. Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample
   
3. Standard dilution buffer 10ml/20ml 4°C
   
4. Biotin-detection antibody (Concentrated) 60ul/120ul 4°C
   
5. Antibody dilution buffer 5ml/10ml 4°C
   
6. HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive)
   
7. SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive)
   
8. Stop solution 5ml/10ml 4°C
   
9. Wash buffer (25X) 15ml/30ml 4°C
   

Protocol Summary

Safety:  Contains sulfuric acid.  TMZ.  Avoid contact.  Wear eye protection, gloves