MOUSE, RAT sAPP ALPHA ELISA Kit (0.6pg/ml Sensitivity, 96T; Soluble Amyloid Precursor Protein Alpha: IBL 27419)

Soluble Amyloid Precursor Protein Alpha (sAPPα) ELISA Kit (Mouse/Rat Specificity) 96T

Sensitive and selective colorimetric ELISA kit to sAPPα with assay range 4.1 - 260 pg/ml, 0.6 pg/ml sensitivity. Highly selective to sAPPα with minimal cross reactivity to sAPPβ. 96T

Specifications:

Complete 96T ELISA kit in sandwich format. Colorimetric detection at 450 nm.
ELISA plate is coated with an affinity purified polyclonal capture antibody that recognizes mouse and rat sAPPα at the "alpha" secretase cleavage site. The detection antibody is also a polyclonal antibody that recognizes rat/mouse sAPPalpha at its N-terminus.
The kit detects sAPPα in EDTA-plasma, serum, cell culture media.
EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
Assay range: 4.1pg/ml - 260 pg/ml, Sensitivity: 0.6pg/ml

Protocol Overview

Protocol

Epitope Positions

^Data

Background on sAPPα

Cleavage of amyloid precursor protein (APP) by “alpha-secretase” creates the extracellular soluble fragment known as sAPPα. Because the “alpha-secretase” cleavage site is within the amyloid-beta molecule, generation of sAPPα by "alpha-secretase" prevents development of fibrillogenic amyloid-beta. The formation of sAPPalpha may therefore be neuroprotective and sAPPalpha may function in neuronal excitability, synaptic plasticity, synaptogenesis, neurite outgrowth, and cell survival. In addition, sAPPalpha appears to modify potassium channels, N-methyl-D-aspartate receptors, and the transcription factor NF kappa B¹.

Methods Overview

Equilibrate plate to RT
Add sample to plate, incubate overnight
Add detection antibody, incubate 1 hr
Add color reagents, 30 min
Add stop solution. Measure OD at 450nm

Storage: 4^oC, 1 year

Safety: Stop solution contains acid. Avoid contact, wear eye protection.

Shipping: Overnight delivery with styrofoam box with ice packs. International delivery is also available: Please inquire for international delivery costs.

kw. sAPP, sAPPa, soluble amyloid precursor protien alpha, mouse, amyloid beta, sAPPalpha, ELISA

Category	Download Link
Protocol Manual	click here
MSDS	click here

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