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FATP5; FATTY ACID TRANSPORTER PROTEIN 5 (55pg/ml Sensitivity) ELISA KIT (Human; bile acyl-CoA synthethase, Part No. hFATP5-ELISA)

FATP5; FATTY ACID TRANSPORTER PROTEIN 5 (55pg/ml Sensitivity) ELISA KIT (Human; bile acyl-CoA synthethase, Part No. hFATP5-ELISA)
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Price: $645.00
Availability: In Stock
Model: hFATP5-ELISA

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Human Fatty Acid Transport Protein 5 ELISA Kit (FATP5; 96T)   

(FATP5, bile acyl-COA Synthethase)

 
Dynamic Assay Range: 0.1ng/ml - 40ng/ml.  Sensitivity: 0.05ng/ml.
Lead Time:  Approximately 1.5 weeks

Fully guaranteed to meet specifications

Specifications
  • Fully pre-configured ELISA kit in sandwich assay format - 96T.
  • Dynamic Assay Range: 0.1ng/ml - 40ng/ml.  Sensitivity: 55 pg/ml.
  • Rapid ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate.
  • Available in 96T format.

Number of Assays
 Cost
96T
 $580
Background
Fatty Acid Transport Protein 5 (FATP5, bile acyl-CoA synthethase) helps to uptake and process long fatty acid chains in liver cells.  FATP5 is only expressed in hepatic cells at the hepatocyte's basal membrane.  FATP5 also help re-conjugate long chain fatty acids to bile molecules.1

1.  Gastroenterology. 2006 Apr;130(4):1245-58.  Targeted Deletion of FATP5 Reveals Multiple Functions in Liver Metabolism: Alterations in Hepatic Lipid Homeostasis.
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
   Sandwich ELISA Methods to FATP5                                                

ELISA Assay Steps
  1. Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.

 

 

Protocol
 

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For research only.  Not for diagnostics.
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4°C. 6 months.
Shipping.  Overnight domestic delivery recommended with ice pack (cost: $60 for 1-2 kits).  Also avaialble for international delivery; please inquire for shipping cost. 
kw: FATP5 ELISA, Fatty acid transport protein Assay


 

Category Download Link
Protocol Manual click here
MSDS click here