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InformationMouse, Rat Amyloid Beta 1-42 ELISA Kit, .05pg/ml Sensitivity (Part 27721)
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Mouse, Rat Amyloid-Beta 1-42 ELISA Kit
Sensitive and selective colorimetric ELISA kit to mouse ABeta (1-42) with sensitivity 0.05pg/ml and 1.56-100 pg/ml assay range. Highly selective to mouse and rat amyloid-beta 1-42 with minimal cross reactivity to human amyloid-beta 1-42 (see data below). The affinity purified monoclonal antibody absorbed on the plate and the affinity purified polyclonal-HRP detection antibody were generated to ABeta 1-42 .
Lead time: Approximately 1 week
Specifications:
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Complete ELISA kit in sandwich format. Colorimetric detection at 450 nm.
- ELISA plate is coated with an affinity purified monoclonal antibody that recognizes mouse and rat amyloid-beta 1-42.
- The kit detects amyloid-beta 1-42 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
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Assay range: 1.56 - 100 pg/ml, 0.05pg/ml sensitivity
- Made in Japan
Protocol Overview
Protocol
Background on Amyloid-Beta (1-42)
Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by certain secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. The prevailing isoforms of Amyloid-Beta are Aβ40 and Aβ42. Aβ40 is the most widespread amyloid-Beta (Aβ) isoform, while Aβ42 is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease. The affinity purified monoclonal antibody percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody in this kit are selective to Aβ42 with insignificant cross-reactivity to Aβ40. The antibodies have been generated to the N-terminus of Aβ42.
Methods Overview-
Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Data Validation
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery in styrofoam box with ice packs. International delivery is also available: please inquire for costs.
