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p65 / RELA Total ELISA Kit (Kits avail for human, mouse, rat: Part hm, ms, rt NFKB-ELISA)

p65 / RELA Total ELISA Kit (Kits avail for human, mouse, rat: Part hm, ms, rt NFKB-ELISA)
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Price: $655.00
Availability: In Stock
Model: hm, ms, rt NFKB-ELISA

DetailsManuals & DownloadsRelated Products
p65 - RelA (NF-kappa B) total ELISA Kits
 
Separate kits are available for human, mouse, rat.  96T
 
Deteects total p65(RelA)
 
Lead time:  2 weeks
.
   
      Protocol Mouse
      Protocol Rat

 

Product name

NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit
Catalogue No.

hm, ms, rt NFKB-ELISA
Alias

NF-κB p65 ELISA Kit, p65RelA ELISA Kit, Nuclear factor NF-kappa-B p65 subunit ELISA Kit
Detection method

Sandwich ELISA, Double Antibody 
Application

NF-κB p65 ELISA Kit allows for the in vitro quantitative determination of NF-κB p65 concentration
Size

96T
Range

0.313-20ng/ml
Sensitivity

< 0.188ng/ml
Standard curve for human

Human NF-κB p65 (Nuclear Factor Kappa B p65) ELISA Kit
Storage

4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of NF-κB p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κB p65 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-99 95
EDTA plasma(n=5) 89-93 90
heparin plasma(n=5) 86-99 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κB p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 86-101% 88-99% 85-99%
EDTA plasma(n=5) 82-100% 87-99% 84-101% 85-100%
heparin plasma(n=5) 87-97% 85-99% 82-93% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%
 
 
Kit Components Item Specifications (48T/96T) 
  1. Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C 
  2. Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample 
  3. Standard dilution buffer 10ml/20ml 4°C 
  4. Biotin-detection antibody (Concentrated) 60ul/120ul 4°C 
  5. Antibody dilution buffer 5ml/10ml 4°C 
  6. HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive) 
  7. SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive) 
  8. Stop solution 5ml/10ml 4°C 
  9. Wash buffer (25X) 15ml/30ml 4°C 

Protocol Summary

1.          Wash plate 2 times before adding standard, sample and control (zero) wells!
2.          Add 100μL standard or sample to each well and incubate for 90 minutes at 37°C
3.          Aspirate and wash plates 2 times
4.          Add 100μL Biotin-labeled antibody working solution to each well and incubate for 60 minutes at 37°C
5.          Add 100 μLSABC Working Solution into each well and incubate for 30 minutes at 37°C
6.          Aspirate and wash plates 5 times
7.          Add 90μL TMB Substrate. Incubate 15 -30 minutes at 37°C
8.          Add 50μL Stop Solution. Read at 450nm immediately
9.          Calculation
 
Shipping:  Overnight, 2-day with ice pack, international shipping 
Storage:  4oC

Safety:  Contains sulfuric acid.  TMZ.  Avoid contact.  Wear eye protection, gloves


 

 

Category Download Link
Protocol Human click here
Protocol Mouse click here
Protocol Rat click here
MSDS click here

 

 

 

 

 



 

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