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InformationCPG Ferrocene | Electrochemical Oligonucleotide Modification Reagent | No. HPT1001
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CPG-Ferrocene (Ecpg-ReporterTM)
Overview:
3’-Ferrocene CPG is a reagent used in oligonucleotide synthesis. Ferrocene CPG is used to attach a Ferrocene molecule at the 3’-end of oligonucleotides. A Ferrocene linked oligonucleotide provides high sensitivity detection with electrochemical techniques such as impedance analysis.
Specifications:
Chemical Name: 1-Dimethoxytrityloxy-3-N-carboxy-Ferrocene- propyl-2-O-succinoyl-long chain alkylamino-CPG
Purity: >98% purity
Appearance: Light yellow solid
Storage: 4oC, desiccate. Ships at ambient temperature
Available Quantities: Use Available Options menu above to order
|
Quantity |
Price |
|
100 mg |
$225 |
|
200 mg |
$325 |
|
1 g |
$950 |
Bulk quantities are also available. Inquire with customersupport@fivephoton.com.
Related Products
CPG-Ferrocene Reaction Scheme:
Additional Notes: CPG-Ferrocene is a 3’-Ferrocene labeled solid support used for labeling oligos from the 3’-end during oligonucleotide synthesis. Compared with Ferrocene NHS Ester conjugation of primary amino-linked oligonucleotides, CPG-Ferrocene provides direct labeling of Ferrocene moieties with the benefit of substantially higher purity and convenience of purification. Labeled ferrocene oligonucleotides are detected by voltammetry.
Coupling: This support should be used in a manner identical to a normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Inquire for COA of current lot
References on Applying Ferrocene as a Molecular Reporter on Oligonucleotides, and Voltammatry Applications:
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Anne A, Bouchardon A, Moiroux J. 2003. 3'-Ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces: novel model systems for exploring flexibility of short DNA using cyclic voltammetry. J Am Chem Soc 125: 1112-3.
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Anne A, Demaille C. 2006. Dynamics of electron transport by elastic bending of short DNA duplexes. Experimental study and quantitative modeling of the cyclic voltammetric behavior of 3'-ferrocenyl DNA end-grafted on gold. J Am Chem Soc 128: 542-57.
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Jenkins DM, Chami B, Kreuzer M, Presting G, Alvarez AM, Liaw BY. 2006. Hybridization probe for femtomolar quantification of selected nucleic acid sequences on a disposable electrode. Anal Chem 78: 2314-8.
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Munoz-Serrano L, Guadalupe AR, Vega-Bermudez E. 2005. Morphological studies of oligodeoxyribonucleotides probes covalently immobilized at polystyrene modified surfaces. J Biotechnol 118: 233-45.
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Radi AE, Acero Sanchez JL, Baldrich E, O'Sullivan CK. 2006. Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. J Am Chem Soc 128: 117-24.
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Wu ZS, Jiang JH, Shen GL, Yu RQ. 2007. Highly sensitive DNA detection and point mutation identification: an electrochemical approach based on the combined use of ligase and reverse molecular beacon. Hum Mutat 28: 630-7.
Shipping and Storage: Ships at ambient temeperature. Store at 4oC, desiccate.
Shipping: Ships at ambient temperature. International delivery is available: please inquire for rate.
kw. electrochemical biosensor, eletrochemical conjugate, electrochemical modification, voltometry
