Canine Amyloid-Beta ELISA Kits (1-40). Part No. c2ABETA1-40-ELISA , kw. beta amyloid, ABeta, AB

Canine Amyloid-Beta ELISA Kits (1-40).  Part No. c2ABETA1-40-ELISA ,  kw. beta amyloid, ABeta, AB
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Price: $645.00
Availability: In Stock
Model: c2ABETA1-40-ELISA

Canine Amyloid-Beta (1-40;  Abeta1-40, AB1-40) ELISA Assay Kit
 
Dimentia Disease and Amyloid Synthesis and Degradation Pathway ELISA Kit Product Line

Highlights
  • Fully pre-configured sandwich ELISA format providing convenience and high sensitivity
  • Strip plate format allowing separate use and storage of ELISA plate sections
  • Affinity purified monoclonal capture antibody on plate.  Separate biotin-conjugated detection antibody
  • Colorimetric detection
  • Rapid Assay: 1.5 hr total incubation at 37oC.
  • Suitable for CSF, other biological fluids, cell and tissue homogenates and lysates
  • ELISA kits are also available for human and rat Abeta1-40;  please inquire.
(Lead time:  2 weeks.  Use the protocol shipped with the kit for your experiment).
 

Specifications:
 
Standard Concentration:  2400 pg/ml
 
Assay Range:  15pg/ml2000 pg/ml
 
Sensitivity:  10.628 pg/ml
 
 

Kit Contents
  1. 8 x 12 well removable section strip plate coated with antibody
  2. Polyclonal detection antibody conjugated with biotin
  3. Steptavidin-HRP
  4. Protein Standard
  5. Standard diluent
  6. Concentrated wash solution
  7. Chromogen solutions A and B
  8. Stop solution
  9. Plate sealers
 
For canine Aß(1-42) ELISA Kit, click here

Background on Protein Targets of the Amyloid ELISA Kits

Amyloid-Beta:  Aged canines accumulate human-type amyloid-beta peptide and undergo declines in cognitive function1,2.  Amyloid-beta concentrations in cerebral-spinal fluid appear to reflect amyloid-beta accumulation in brain1,2.   Amyloid-beta peptides, and secretases in canine are similar to human1.  Therefore, canine amyloid-beta accumulation and decline in cognitive function are being employed as an animal model for the onset of dimentia disease in humans. 

Amyloid-Beta (Aβ) is generated by sequential cleavage by beta and gamma secretases of the amyloid precursor protein (APP), a transmembrane protein of unknown function.  Inhibition of secretase activity has been proposed as a therapeutic strategy to limit amyloid-Beta (Aβ) accumulation. The γ secretase, which produces the C-terminal end of the Aβ peptide, cleaves within the transmembrane region of APP and generates amyloid-Beta (Aβ) isoforms of 36-43 amino acid residues in length.  The most common isoforms of amyloid-Beta are Aβ40 and Aβ42.  The shorter amyloid-Beta isoform is produced by cleavage in the endoplasmic reticulum, while the longer isoform is processed in the trans-Golgi network.  Aβ40 is the most common amyloid-Beta (Aβ) isoform, but Aβ42 is the more fibrillogenic and thus the predominant form associated Alzheimer’s disease.  Antibodies employed in this Amyloid-Beta ELISA kit are selective for the Aβ42 isoform of amyloid-beta, with insignificant cross-reactivity to the Aβ40isoform.

Background References:
  1. Head E, Pop V, Sarsoza F, Kayed R, Beckett TL, Studzinski CM, Tomic JL, Glabe CG, Murphy MP.  2010.  Amyloid-beta peptide and oligomers in the brain and cerebrospinal fluid of aged canines.  J Alzheimers Dis. 2010;20(2):637-46.
  2. Cummings BJ,  Su JH, Cotman CW, White R, Russel MJ.  1993.  Beta-amyloid accumulation in aged canine brain: a model of early plaque formation in Alzheimer's disease. Neuro Aging. 1993 Nov-Dec;14(6):547-60.

Methods Overview:

The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.  
 
 
 

  Representative Sandwich ELISA Methods   

                                                        ELISA Assay Steps

 

  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.
 
 
 

Safety, Storage, Shipping
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC, 6 months.  Strip plate sections, standard, and other solutions can be stored longer term at -20oC.
Shipping.  Overnight domestic delivery recommended.  Available for international shipping.
 
 
 
 
 
 

 


 

Category Download Link
Protocol Manual click here
MSDS click here