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InformationRat Rantes ELISA Kit (Part FPEK0496)
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Rat Rantes ELISA Kit (96T)
Highly sensitive kit to rat rantes. Validated for sensitive and selective detection in cell culture medium, serum and plasma.
Currently unavailable. Call for availability of substitute kit to same target
Highlights
- Fully configured colorimetric ELISA kit in sandwich fomat.
- Natural - purified protien provided as standard.
- Assay range: 15.6pg/ml-1000pg/ml.
- Validated for cell culture medium, serum and plasma.
View protocolKit Contents
| Description | Quantity |
| 96- well plate precoated with anti-rat Rantes antibody | 1 |
| Lyophilized natural rat Rantes standard | 10ng (1 vial) |
| Biotinylated anti-rat Rantes Antibody | 130 ul (dilution 1:100) |
| Sample diluent buffer |
30 ml |
| Antibody diluents buffer |
12 ml |
| ABC diluents buffer |
12 ml |
| TMB color developing agent |
10 ml |
| TMB stop solution |
10 ml |
Rantes ELISA Kits Availability And Specifications
|
Species |
Assay Range |
| Human | 15.6pg/ml-1000pg/ml |
| Rat | 15.6pg/ml-1000pg/ml |
| Mouse | 15.6pg/ml-1000pg/ml |
Use Available Options menu to select the species specificity of the Ratnes ELISA Kits.
Background on Rantes
Rantes is a protein also known as CCL5 and is encoded by the CCL5 gene. Rantes is classified as a chemotactic cytokine, and is chemotactic for T cells, eosinophils, basophils, and recruits leukocytes in response to inflammation. Rantes induces proliferation and activation of natural killer cells to form CHAK cells. It is also an HIV-supressive factor.
Methods Overview: Sandwich ELISA
This rat Rantes ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Rantes is precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rantes is added subsequently and then followed by washes with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rantes concentration captured on the plate.
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Representative Sandwich ELISA Methods |
- Add sample to plate, incubate 90 min, 37oC.
- Add detection antibody, incubate 1 hr, 37oC.
- Add ABC solution, incubate 30 min 37oC.
- Add chromogens, incubate in dark for 25 min.
- Add stop solution. Measure OD at 450nm.
References
1. Liu, H.; Chao, D.; Nakayama, E. E.; Taguchi, H.; Goto, M.; Xin, X.; Takamatsu, J.; Saito, H.; Ishikawa, Y.; Akaza, T.; Juji, T.; Takebe, Y.; and 9 others. Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression. Proc. Nat. Acad. Sci. 96: 4581-4585, 1999.
2. Schall, T. J.; Jongstra, J.; Dyer, B. J.; Jorgensen, J.; Clayberger, C.; Davis, M. M.; Krensky, A. M. A human T cell-specific molecule is a member of a new gene family. J. Immun. 141: 1018-1025, 1988.
Safety: Stop solution contains acid. Avoid eye contact. Wear eye protection and gloves.
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.
Shipping. Overnight delivery