Human APLP2 (Amyloid-Like Protein 2; Part APLP2-ELISA

Human APLP2 (Amyloid-Like Protein 2) ELISA Kit (96T)

Introduction

APLP2 plays a role in synaptic plasticity, functioning to promote neurite outgrowth, neural cell migration and copper homeostasis.  Analysing the neurons and networks of APP/APLP2 double knock out mice using stem cell-derived neurons and slice cultures, shows deficient excitatory synaptic transmission in this genotype.  Moreover, APLP2 together with APP has been demonstrated to exhibit presynaptic and postsynaptic functions in synaptogenesis  and maintenance of synapses.

APLP2 has shown to act as a cargo receptor in axonal transport for intact proteins.

APLP2 is part of a family of mammalian membrane proteins along with APLP1 and amyloid precursor protein (APP).  Since APP plays a key role in the molecular pathology of Alzheimer’s disease (AD), it has been hypothesized that APLP2 also plays a role in AD pathogenesis. The amyloid β peptide (Aβ) that is present on APP has been shown to cause neurotoxic effects leading to AD^.  Although the Aβ sequence is not present on APLP2, it has been suggested that APLP2 and APP share a functional redundancy whereby both proteins interplay with one another to exhibit physiological functions to do with synapse formation.

Protocol

Product name	APLP2 (Amyloid-like protein 2) ELISA Kit
Catalogue Nos.	APLP2-ELISA
Alias	APLP2 ELISA Kit
Detection method	Sandwich ELISA, Double Antibody
Size	96T
Range	0.156-10ng/ml
Sensitivity	< 0.094ng/ml
Standard curve	Human APLP2(Amyloid-like protein 2) ELISA Kit
Storage	4 ℃ for 6 months
Recovery	Matrices listed below were spiked with certain level of and the recovery rates were calculated by comparing the measured value to the expected amount of in samples. Matrix Recovery range(%) Average(%) serum (n=5) 86-103 94 EDTA plasma (n=5) 87-105 97 heparin plasma (n=5) 87-101 97
Linearity	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum (n=5) 90-101% 85-104% 92-102% 88-98% EDTA plasma (n=5) 83-101% 84-99% 91-99% 91-97% heparin plasma (n=5) 82-99% 91-97% 85-99% 80-97%
CV(%)	Intra-Assay: CV<8% Inter-Assay: CV<10%
Note	For Research Use Only

1. Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C
   
2. Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample
   
3. Standard dilution buffer 10ml/20ml 4°C
   
4. Biotin-detection antibody (Concentrated) 60ul/120ul 4°C
   
5. Antibody dilution buffer 5ml/10ml 4°C
   
6. HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive)
   
7. SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive)
   
8. Stop solution 5ml/10ml 4°C
   
9. Wash buffer (25X) 15ml/30ml 4°C
   

Protocol Summary