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PHOSPHATASE INHIBITOR COCKTAIL (100X, WATER SOLUBLE, BROAD SPECTRUM; Potent & Broad Spectrum. Part No. PIC1)

PHOSPHATASE INHIBITOR COCKTAIL (100X, WATER SOLUBLE, BROAD SPECTRUM; Potent & Broad Spectrum. Part No. PIC1)
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Price: $190.00
Availability: In Stock
Model: PIC1
Manufacturer: Fivephoton Biochemicals

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Phosphatase Inhibitor Cocktail
(Water soluble, 100X)
  • Available in 1 ml or 5 ml stock 100X water soluble phosphatse inhibitor cocktail solution. 
  • Add directly to cell lysates or culture medium.  
  • Broad spectrum, highly potent:  Inhibits seryl, threonyl, tyrosyl, alkaline and Ca2+-phosphatases
  • Contains activated activated sodium orthovanadate*, sodium tartrate, sodium fluoride, beta-glycerophosphate.
  • Ships at ambient temperature.  Store at 4oC short term (4 months) or -20oC long term (2 years).

Cost
Volume
$190
1 ml
$320
5 ml

Use Available Options menu to order.


  Contents
  Target
Sodium Orthovanadate Activated (100mM)*
Tyr and alkaline phosphatase inhibitor
   Sodium Fluoride (500mM)
  Acid phosphatase inhibitor
   beta-glycerophosphate (300mM)
 Ser/Thr phosphatase inhibitor
   Sodium Tartrate (400mM)
  Acid Phosphatase inhibitor

*Activated sodium orthovandate:  Prepared by adjusting to pH 10, and heating-cooling cycles until the solution maintains a colorless appearance at equilibrium at pH 10.  Activated sodium orthovanadate is prepared by the protocol described in: Gordon, J.: Methods Enzymol. (1991) 201, 477-482.



Image:  Crystal Structure of Human Dual Specificity Phosphatase 14 (Source: PDB)
Instructions:  Phosphatase Inhibitor Cocktail

1.  Dispense phosphatase inhibitor cocktail directly into cell lysates using a 1/100 dilution.

Product References

1.  Park, Kyeong-Min, Ji-Young Park, Jaehyuk Pyo, Sun-Young Lee, and Ho-Shik Kim. 2022. "Induction of DR5-Dependent Apoptosis by PGA2 through ATF4-CHOP Pathway" Molecules 27, no. 12: 3804. https://doi.org/10.3390/molecules27123804
2.  Christopher R. Donnelly, et al. 2021. STING controls nociception via type I interferon signalling in sensory neurons. Nature volume 591,  275–280.  Link to article
3. Joe Truong Nguyen,  (2018).  Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration. Science Advances  09 May 2018:  Vol. 4, no. 5, eaao5838.  Link to article
4  Satyanarayanan, S.K., Shih, YH., Chien, YC. et al. Mol Neurobiol (2018) 55: 7271. https://doi.org/10.1007/s12035-018-0899-x.  Link to article
5  Villa‐Diaz, L. G., Kim, J. K., Laperle, A., Palecek, S. P., & Krebsbach, P. H. (2016). Inhibition of FAK signaling by integrin α6β1 supports human pluripotent stem cell self‐renewal. STEM CELLS.
6.  Wang, J. T., et al. "Src controls neuronal migration by regulating the activity of FAK and cofilin." Neuroscieince 292 (2015): 90-100.
References on Phosphatase Inhibition
  1. Arnold WN, Sakai KH, and Mann LC. Selective inactivation of an extra-cytoplasmic acid phosphatase of yeast-like cells of Sporothrix schenckii by sodium fluoride. J Gen Microbiol 133: 1503-1509, 1987.
  2. Botana LM, Alfonso A, Botana MA, Vieytes MR, Louzao MC, and Cabado AG. Influence of protein kinase C, cAMP and phosphatase activity on histamine release produced by compound 48/80 and sodium fluoride on rat mast cells. Agents Actions 37: 1-7, 1992.
  3. Gordon JA. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol 201: 477-482, 1991.
  4. Guideri R, Cesari L, Burghesi R, and Medaglini E. [Behavior of calcemia, phosphoremia, calciuria, phosphaturia and alkaline phosphatase in normal subjects treated with sodium fluoride (NaF)]. Boll Soc Ital Biol Sper 42: 1612-1615, 1966.
  5. Lange AJ, Arion WJ, Burchell A, and Burchell B. Aluminum ions are required for stabilization and inhibition of hepatic microsomal glucose-6-phosphatase by sodium fluoride. J Biol Chem 261: 101-107, 1986.
  6. Li L, Eisen AZ, Sturman E, and Seltzer JL. Protein tyrosine phosphorylation in signalling pathways leading to the activation of gelatinase A: activation of gelatinase A by treatment with the protein tyrosine phosphatase inhibitor sodium orthovanadate. Biochim Biophys Acta 1405: 110-120, 1998.
  7. Suketa Y, Kuwahara M, Sahashi K, Suzuki N, and Akiyama Y. A role of renal K+-stimulated phosphatase activity on elevation of urinary sodium excretion in fluoride-treated rats. Toxicol Appl Pharmacol 69: 225-233, 1983.
Phosphatase Inhibitor Cocktail:  Safety, Storage, Shipping
Safety:  Phosphatase Inhibitor Cocktail PIC1:  Harmful;  avoid ingestion, eye and skin contact.
Storage:  Phosphatase Inhibitor Cocktail PIC1:  Stable at 4oC for 4 months.  For prolonged storage, store at -20oC.

Shipping: Ships at ambient temperature.  2-day or overnight domestic delivery.  International delivery is also available. 

KW PHOSPHATASE INHIBITOR, SODIUM FLUORIDE, SODIUM ORTHOVANADATE, BETA-GLYCEROPHOSPHATE, SODIUM TARTRATE




 

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