MTT Cell Proliferation, Viability and Toxicity Assay Kit (Cyto-M; Part No. MTT-1200)

For 1200 Assays,based on one assay per well in a 96-well dish.

• Colormetric detection method to quantitate cell viability.
• 96-well format.  Scalable to other sized sample wells.
• Easily adapted to measure cell proliferation.
• Representative data displayed in Images and Data tab.

View Protocol

Related Product of Interest:  Highly sensitive cell counting, cell proliferation and cell death assay that uses WST-8 reagent.

Fivephoton Cell Viability and Proliferation Assay Kit:  click here

The Cyto-M^TM MTT Cell-Based Cytotoxicity Kit contains pre-configured reagents to rapidly quantitate cell number spectrophotometrically as a function of mitochondrial activity in living cells.  This kit provides an alternative method to cell counting, and can be applied to most measurements of cell death or cell proliferation.  The Cyto-M^TM MTT Cell-based Toxicity Assay System kit yields accurate and reproducible data by using only three components.  Furthermore, the kit was designed to create minimal disturbances to cells and limits detachment by not requiring frequent rinsing.  The assay is also applicable in bacteria and fungus.

Product Reference

Tang, B., Chow, J. Y., Dong, T. X., Yang, S. M., Lu, D. S., Carethers, J. M., & Dong, H. (2016). Calcium sensing receptor suppresses human pancreatic tumorigenesis through a novel NCX1/Ca 2+/β-catenin signaling pathway. Cancer letters, 377(1), 44-54.

References on Cytotoxicity and Proliferation Assays.

1. Cabrera M, Lavaggi ML, Hernandez P, Merlino A, Gerpe A, et al. 2009. Cytotoxic, mutagenic and genotoxic effects of new anti-T. cruzi 5-phenylethenylbenzofuroxans. Contribution of phase I metabolites on the mutagenicity induction. Toxicol Lett 190: 140-9.
2. Cardin GB, Mantha M, Jumarie C. 2009. Resistance to cadmium as a function of Caco-2 cell differentiation: role of reactive oxygen species in cadmium- but not zinc-induced adaptation mechanisms. Biometals 22: 753-69.
3. Caruso M, Mariotti A, Zizzadoro C, Zaghini A, Ormas P, et al. 2009. A clonal cell line (BME-UV1) as a possible model to study bovine mammary epithelial metabolism: metabolism and cytotoxicity of aflatoxin B1. Toxicon 53: 400-8.
4. Cavalcanti BC, Bezerra DP, Magalhaes HI, Moraes MO, Lima MA, et al. 2009. Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells. J Appl Toxicol 29: 560-8.
5. Cen L, Hutzen B, Ball S, DeAngelis S, Chen CL, et al. 2009. New structural analogues of curcumin exhibit potent growth suppressive activity in human colorectal carcinoma cells. BMC Cancer 9: 99
6. Chang MC, Lin LD, Chan CP, Chang HH, Chen LI, et al. 2009. The effect of BisGMA on cen species- and MEK/ERK-dependent and -independent pathways. Biomaterials 30: 4070-7.
7. Charupant K, Daikuhara N, Saito E, Amnuoypol S, Suwanborirux K, et al. 2009. Chemistry of renieramycins. Part 8: synthesis and cytotoxicity evaluation of renieramycin M-jorunnamycin A analogues. Bioorg Med Chem 17: 4548-58.