Human Klotho (KL) ELISA Kit (Part hKL-ELISA)

Human Klotho (KL) ELISA Kit

Product name	Human KL(Klotho) ELISA Kit
Catalogue No.	hKL-ELISA
Alias	KL ELISA Kit, klotho ELISA Kit, KLOTHO ELISA Kit
Detection method	Sandwich ELISA, Double Antibody
Application	KL ELISA Kit allows for the in vitro quantitative determination of KL concentrations in plasma, tissue homogenates and other biological fluids.
Size	96T
Range	7.8-500pg/ml
Sensitivity	< 4.688pg/ml
Standard curve	Human KL(Klotho) ELISA Kit
Storage	4 ℃ for 6 months
Recovery	Matrices listed below were spiked with certain level of KL and the recovery rates were calculated by comparing the measured value to the expected amount of KL in samples. Matrix Recovery range(%) Average(%) serum(n=5) 90-103 96 EDTA plasma(n=5) 88-104 96 heparin plasma(n=5) 88-103 95
Linearity	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 1:16 serum(n=5) 86-95% 88-104% 85-97% 85-105% EDTA plasma(n=5) 83-100% 93-101% 90-101% 86-100% heparin plasma(n=5) 80-99% 83-97% 81-97% 81-95%
CV(%)	Intra-Assay: CV<8% Inter-Assay: CV<10%

1. Storage Micro ELISA Plate (Dismountable) 8×6 or 8×12 4°C/-20°C 
   
2. Lyophilized Standard 1 vial or 2 vial 4°C/-20°C Sample 
   
3. Standard dilution buffer 10ml/20ml 4°C 
   
4. Biotin-detection antibody (Concentrated) 60ul/120ul 4°C 
   
5. Antibody dilution buffer 5ml/10ml 4°C 
   
6. HRP-Streptavidin Conjugate(SABC) 60ul/120ul 4°C (light sensitive) 
   
7. SABC dilution buffer 5ml/10ml 4°C TMB substrate 5ml/10ml 4°C (light sensitive) 
   
8. Stop solution 5ml/10ml 4°C 
   
9. Wash buffer (25X) 15ml/30ml 4°C 
   

Protocol Summary