MOUSE / RAT AMYLOID BETA ELISA KIT (1.56-100 pg/ml assay range, AB1-42, amyloid beta, abeta, 42); IBL 27721

Mouse / Rat Amyloid-Beta 1-42 ELISA Kit (96T)

This highly validated, sensitive and selective Mouse / Rat Amyloid Beta (1-42) ELISA kit measures rat and mouse Amyloid Beta 1-42 in serum or EDTA plasma with an assay range of 1.56 - 100 pg/mL (0.35 - 22.6 pmol/L);  0.5 pg/ml sensitivity.  96T

Lead time estimate:  2 weeks

Specifications:

1. Complete ELISA kit in sandwich format.  Colorimetric detection at 450 nm.  96T
2. ELISA plate is coated with an affinity purified monoclonal antibody that recognizes mouse/rat amyloid-beta 1-42.
3. The kit detects amyloid-beta 1-42 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
4. EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
5. Incubation times:  Samples with plate;  overnight.  Detection antibody:  1 hr
6. Assay range:  1.56 - 100 pg/ml, 0.5 pg/ml sensitivity
   

  Protocol

 Epitope Positions

Background: 
Amyloid Beta proteins are the main component of the plaque found in the brains of Alzheimer's patients.  Amylid beta proteins are usually 40 - 43 amino acids long.  They are cleaved from the much longer (695, 751, or 770 amino acids long) Amyloid Precursor Protein (APP).  The accumulation of amyloid-beta 42 has been proposed as an underlying mechanism for Alzheimer's disease.

Methods Overview: 

1. Equilibrate plate to RT
2. Add sample to plate, incubate overnight
3. Add detection antibody, incubate 1 hr
4. Add color reagents, 30 min
5. Add stop solution.  Measure OD at 450nm 

Validation Data:  To enlarge, right click view image.

References:

1. Selkoe DJ. Normal and abnormal biology of the beta-amyloid precursor protein. Annu Rev Neurosci. 1994;17:489-517.
2. Wang R, Sweeney D, Gandy SE, Sisodia SS. The profile of soluble amyloid beta protein in cultured cell media. Detection and quantification of amyloid beta protein and variants by immunoprecipitation-mass spectrometry. J Biol Chem. 1996 Dec 13;271(50):31894-902.
3. Saido TC, Iwatsubo T, Mann DM, Shimada H, Ihara Y, Kawashima S. Dominant and differential deposition of distinct beta-amyloidpeptide species, A beta N3(pE), in senile plaques. Neuron. 1995 Feb;14(2):457-66.