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Fivephoton Cell Proliferation, Viability and Density Measuring Kit, High Sensitivity

Fivephoton Cell Proliferation, Viability and Density Measuring Kit, High Sensitivity
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Price: $139.00
Availability: Out Of Stock
Model: FCVK-500

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Fivephoton Cell Proliferation, Viability and Density Measuring Kit

This cell proliferation, viability and density measuring kit is applicable to 500 assays using cells plated in 96-well dishes

Highliglhts

  1. Requires only three assay steps.
  2. Measures cell viability by a chemical reaction involving cellular dehyrogenase activity and reduction of the water soluble substrate WST-8 into the colored product formazan that absorbs at 450nm.
  3. The cell proliferation and viability assay uses a relatively short incubation time and has minimal toxicity toward cells relative to similar kits.
  4. Higher sensitivity than related assays, including MTT, XTT, MTS and WST-1.
  5. Measure cell viability and proliferation in high-throughput format in mutliwell dishes.



Number of Kits
Number of Assays (in 96T wells)
Price
1
500
$139
2
1000
$234
4
2000
$409

Protocol Synopsis for Fivephoton Cell Viability and Proliferation Assay

  1. Plate cells in 96-wells dishes and culture until approximately 85% confluency.   (Other mutliwell dishes are acceptable as well).  Use "control"  cells for baseline comparison.
  2. Add WST-8 reagent.
  3. Incubate cells 1-4 hours.
  4. Measure OD at 450 nm.
  5. The relative absorbance at 450 nm provides an index of viable cell number.

Reaction scheme involving NADPH and conversion of colorless WST-8 into its orange reduced form.

 
Fig. 1
Cellular dehydrogenases convert the colorless substrate WST-8 to colored formazan that is measureable at 450 nm serving as the quantitative basis for the assay.









Fig. 2
WST-8 reagent, which is the substrate in the Fivephoton Cell Viability and Proliferation Kit, is substantially more sensitive than other reagents employed in cell proliferation assays.
Fig. 3
Measuring Proliferation and Cytotoxicity after treatments with IL-2 and TNF-alpha, respectively.



















 
Fig 4
Comparisons in Cell Viability and Proliferation Reagent Handling.  WST-8 requires fewer steps to assay.

The Fivephoton Cell Viability and Proliferation Assay Kit is a colorimetric assay for determining the number of viable cells in proliferation or cytotoxicity assays that uses the reagent WST-8 as its substrate. The highly water-soluble tetrazolium salt, WST-8 is cleaved to a soluble formazan by cellular dehydrogenases. This bioreduction is accomplished by NADPH or NADH produced by dehydrogenase enzymes in metabolically active cells. The amount of formazan dye product directly correlates to the number of metabolically active cells in the culture.  Because the WST-8 formazan dye is soluble in tissue culture medium, the Fivephoton Cell Viability and Proliferation Assay Kit requires fewer steps than procedures that use the tetrazolium compound MTT.  The results  obtained  by using  the  kit  show  higher  sensitivity  than  methods  that  use tetrazolium compounds such as MTT, MTS, XTT or WST-1.

The ready-to-use WST-8 solution does not require additional steps such as thawing before use.  Therefore, the storage stability of the Fivephoton Cell Viability and Proliferation kit is higher than kits using other tetrazolium compounds.
Safety:  Cell proliferation and viability assay:  Avoid contact, wear eye protection
Shipping:  Ambient temperature, overnight and 2-day delivery
Storage:  4oC for at least one year.  Extended storage at -20oC.


 

Category Download Link
Protocol Manual click here
MSDS click here



 

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