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InformationSUMO2 (Human SUMO 2, Sentrin-2, Small ubiquitin-Related Modifier 2) ELISA Kit (Part hSUMO2ELISA)
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Human SUMO2 ELISA Kit
Small Ubiquitin-Related Modified 2 Protein ELISA Kit
Dynamic Assay Range: 200pg/ml - 60ng/ml. Sensitivity: 155 pg/ml.
Lead Time: Approximately 1.5 weeks
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Number of Assays
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Cost
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96T
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$655
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2x96T
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$1198
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Highlights
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Intoduction
to Sumo 2
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SUMO 2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin, which targets proteins for degradation, SUMO2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.
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Methods
Overview
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The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
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Sandwich ELISA Methods to SUMO2
ELISA Assay Steps
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Mix sample, biotinylated-detection antibody and streptavidin-HRP.
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Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.Wash 5 times.
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Apply chromogens, incubate, then stop reaction.
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SUMO2
References
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For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4°C. 6 months.
Shipping. Overnight domestic delivery recommended with ice pack (cost: $52 for 1-2 kits). Also avaialble for international delivery; please inquire for shipping cost.
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