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SUMO1 (Small Ubiquitin-Related Modifier 1) 150pg/ml-40ng/ml Assay Range ELISA Kit (Human, 96T, Part hSUMO1ELISA-Biotin)

SUMO1 (Small Ubiquitin-Related Modifier 1) 150pg/ml-40ng/ml Assay Range ELISA Kit (Human, 96T, Part hSUMO1ELISA-Biotin)
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Price: $655.00
Availability: In Stock
Model: hSUMO1ELISA-biotin

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DetailsManuals & DownloadsRelated Products
Human SUMO1 ELISA Kit
 
Small Ubiquitin-Related Modified 1 Protein ELISA Kit
Dynamic Assay Range: 150pg/ml - 40ng/ml.  Sensitivity: 128 pg/ml.
Lead Time:  Approximately 1.5 weeks   
 
   
Number of Assays
  Cost 
96T
$655
96T
$1198
     
 
 
 
 
 
 
Highlights
 
  • Fully pre-configured ELISA kit in sandwich assay format.
  • Dynamic Assay Range: 150pg/ml - 40ng/ml.  Sensitivity: 128pg/ml.
  • Standard peptide concentration:  48ng/ml
  • Rapid and simple ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with biotinylated detection antibody and streptavidin-HRP prior to addition to the plate.
  • Detects free and protein-conjugated SUMO1 in cell lysates, and nuclear and cytoplasmic fractions.

 
Intoduction to Sumo  1
 
 
 
 
 
 
 
SUMO 1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system.  SUMO1 is involved in a variety of cellular processes, including nuclear transport, transcriptional regulation, apoptosis, and protein stability.  SUMO1 becomes functionally active when the last four amino acids of the carboxy-terminus are cleaved.  SUMO 1 is also associated with SERCA2A, the sarco/endoplasmic reticulum Ca2+ ATPase that regulates intracellular calcium levels.  SUMO 1 activates and stabilizes SERCA2A by binding  to lysine resides 480 and 585.   This ELISA kit detects free SUMO1 and conjugated SUMO1.

 
Methods        
Overview
      
 
 
 
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody.   Chromogen solutions are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.

 

   Sandwich ELISA Methods to SUMO1                                                                                                                                                                                                                   
 
 
ELISA Assay Steps
  1. Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.
 
 
 
      
 
SUMO1             
References        
 
 
 
 
 
 
 
 
    
 
 
 
 
  1. Shen Z, Pardington-Purtymun PE, Comeaux JC, Moyzis RK, Chen DJ (January 1997). "UBL1, a human ubiquitin-like protein associating with human RAD51/RAD52 proteins". Genomics 36 (2): 271–9. doi:10.1006/geno.1996.0462. PMID 8812453.
  2. Okura T, Gong L, Kamitani T, Wada T, Okura I, Wei CF, Chang HM, Yeh ET (Dec 1996). "Protection against Fas/APO-1- and tumor necrosis factor-mediated cell death by a novel protein, sentrin". J Immunol 157 (10): 4277–81. PMID 8906799.
  3. Entrez Gene: SUMO1 SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)".
  4. Pauws E, Stanier P (December 2007). "FGF signalling and SUMO modification: new players in the aetiology of cleft lip and/or palate". Trends Genet. 23 (12): 631–40. doi:10.1016/j.tig.2007.09.002. PMID 17981355.
  5. Dixon MJ, Marazita ML, Beaty TH, Murray JC (2011). "Cleft lip and palate: understanding genetic and environmental influences". Nature Reviews Genetics (12) 167-178.
  6. Tatham, Michael H; Kim Suhkmann, Yu Bin, Jaffray Ellis, Song Jing, Zheng Jian, Rodriguez Manuel S, Hay Ronald T, Chen Yuan (August 2003). "Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation". Biochemistry (United States) 42 (33): 9959–69. doi:10.1021/bi0345283. ISSN 0006-2960. PMID 12924945.
 
For research only.  Not for diagnostics.
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4°C. 6 months.
Shipping.  Overnight domestic delivery recommended with ice packs.  Also avaialble for international delivery; please inquire for shipping cost. 


 

Category Download Link
Protocol Manual click here
MSDS click here



 

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