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InformationRAT SNCA, ALPHA-SYNUCLEIN, 100PG/ML-40NG/ML ASSAY RANGE, ELISA KIT (Part SNCa-Biotin) kw: alpha synuclein,
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Rat Alpha-Synuclein (SNCa) ELISA Kit
Dynamic Assay Range: 100pg/ml - 40ng/ml. Sensitivity: 0.08ng/ml.
Lead Time: Approximately 1.5 week
Specifications
Protocol
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
Sandwich ELISA Methods to Alpha Synuclein (SNCa) 
ELISA Assay Steps
Introduction to Alpha Synuclein:
Alpha-synuclein is a 140 amino acid protein expressed in brain that is associated with fibrillar structures in Lewy Bodies. Alpha-synuclein binds to phospholipid and may assemble into pore-like structures in cellular membranes, but it is primarily located in the cytosol. Generation of aggregates in Lewy Bodies and/or association of mutant or wild-type alpha-synuclein with membraneous structures have been linked to both early-onset congenital and age-related Parkinson’s Disease. Parkinson's disease is a neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features of the disease include postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in brain and spinal cord.
Gerneral References on Alpha Synuclein
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4°C. 6 months.
Shipping. Overnight domestic delivery recommended with ice pack (cost: $42 for 1-2 kits). Also avaialble for international delivery; please inquire for shipping cost.
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