Mouse Kallikrein-6 (KLK6) ELISA Kit (Part mKLK6-Biotin) kw: kallikrein 6, neurosin

Mouse Kallikrein-6 (KLK6) ELISA Kit

Dynamic Assay Range: 200pg/ml - 40ng/ml. Sensitivity: 0,126ng/ml.

Lead Time: Approximately 1.5 weeks

Specifications

Fully pre-configured ELISA kit in sandwich assay format.
Dynamic Assay Range: 200pg/ml - 40ng/ml. Sensitivity: 126pg/ml.
Rapid ELISA Protocol: Requires one 60 min incubation period at 37^oC.
Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate.
Available in 48T and 96T formats.
48T Kit Cost: $355. 96T Kit Cost: $635. 2x96T Kits Cost: $1150

Number of Assays	Cost
48T	$355
96T	$635
2 x 96T	$1150

Methods Overview:

The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.

Protocol

Sandwich ELISA Methods to Kallikrein-6 (KLK6) M

Rapid Protocol ELISA Assay Steps

Mix sample, biotinylated-detection antibody and streptavidin-HRP.
Apply sample and antibody-HRP complex to ELISA plate for 60min at 37^oC. Afterwards, wash 5 times.
Apply chromogens, incubate, then stop the reaction.

Introduction to Kallikrein-6:

Kallikrein-6 is a protein with serine protease activity that is detected with grey matter neurons and colocalizes with pathological inclusions. Kallikreins have many known functions; they are associated with carcinogenisis and are thought to be disease biomarkers. Kallikrein-6 has been found to cleave amyloid precursor protein as well as myelin basic protein, gelatin, casein, and many extracellular matrix proteins. Kallikrein-6 also cleaves alpha-synuclein, preventing polymerization.

Gerneral References on Kallikrein-6

Ashby EL, Kehoe PG, Love S. Kallikrein-related peptidase 6 in Alzheimer’s disease and vascular dementia. Brain Research 1363: 1-10, 2010.
Diamandis EP, Yousef GM, Luo LY, Magklara A, Obiezu CV. The new human kallikrein gene family: implications in carcinogenesis. Trends in endocrinology and metabolism TEM 11: 54-60, 2000.
Magklara A, Mellati AA, Wasney GA, Little SP, Sotiropoulou G, Becker GW, Diamandis EP. Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Biochemical and Biophysical Research Communications307: 948-955, 2003.
Menendez-Gonzalez M, Castro-Santos P, Suarez A, Calatayud MT, Perez-Pinera P, Martinez M, Ribacoba R, Gutierrez C. Value of measuring plasmatic levels of neurosin in the diagnosis of Alzheimer’s disease. Journal of Alzheimers disease JAD 14: 59-67, 2008.
Ogawa K, Yamada T, Tsujioka Y, Taguchi J, Takahashi M, Tsuboi Y, Fujino Y, Nakajima M, Yamamoto T, Akatsu H, Mitsui S, Yamaguchi N. Localization of a novel type trypsin-like serine protease, neurosin, in brain tissues of Alzheimer’s disease and Parkinson's disease.Psychiatry and Clinical Neurosciences 54: 419-426, 2000.
Okui A, Kominami K, Uemura H, Mitsui S, Yamaguchi N. Characterization of a brain-related serine protease, neurosin (human kaillikrein 6), in human cerebrospinal fluid. NeuroReport 12: 1345-1350, 2001.
Shan SJC, Scorilas A, Katsaros D, Diamandis EP. Transcriptional upregulation of human tissue kallikrein 6 in ovarian cancer: clinical and mechanistic aspects. British Journal of Cancer 96: 362-372, 2007.

For research only. Not for diagnostics.

Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.

Storage: 4°C. 6 months.

Shipping. Overnight domestic delivery recommended. Also avaialble for international delivery; please inquire for shipping cost.

Category	Download Link
Protocol Manual	click here
MSDS	click here

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