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InformationHuman BACE1 ELISA Kit (Human Beta-site APP Cleaving Enzyme 1, Part No. 27752 IBL: kw. BACE1
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BACE1 (β-Site APP Cleaving Enzyme 1) ELISA Kit (Human Specificity; 96T)
Sensitive and selective colorimetric ELISA kit to BACE1 with assay range 1.56-100 ng/ml. Highly selective to BACE1 with minimal cross reactivity to BACE1 (1-460) (see data below). The affinity purified monoclonal antibody absorbed on the plate and the affinity purified polyclonal-HRP detection antibody were generated to BACE1. 96T
Specifications:
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Complete ELISA kit in sandwich format. 96T, colorimetric detection at 450 nm.
- ELISA plate is coated with an affinity purified monoclonal antibody that recognizes BACE1.
- The kit detects BACE1 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
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Assay range: 1.56 - 100 ng/ml. 0.16ng/ml sensitivity.
Protocol Overview
Protocol
Background on BACE1
Amyloid-beta (Abeta) deposition in the cerebral cortex is greatly associated with Alzheimer's disease. It is generated through amyloid precursor protein (APP) cleavage by certain secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. The most widespread β-secretase that cleaves APP is known as β-site APP Cleaving Enzyme 1, or BACE1. The affinity purified monoclonal antibody percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody in this kit are selective to BACE1 with insignificant cross-reactivity to BACE1 (1-460).
Methods Overview-
Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
BACE1 ELISA Kit Validation Data
References
- Kitazume-Kawaguchi S, Dohmae N, Takio K, Tsuji S, Colley KJ. The relationship between ST6Gal I Golgi retention and its cleavage-secretion : Glycobiology. 1999 Dec;9(12):1397-406.
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Kitazume S, Tachida Y, Oka R, Shirotani K , Saido TC , Hashimoto Y. Alzheimer's beta-secretase, beta-site amyloid precursor protein-cleaving enzyme, is responsible for cleavage secretion of a Golgi-resident sialyltransferase. : Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13554-9.
- Kitazume S, Tachida Y, Oka R, Kotani N, Ogawa K, Suzuki M, Dohmae N, Takio N, Saido TC, Hashimoto Y. Characterization of alpha 2,6-Sialyltransferase Cleavage by Alzheimer's beta -Secretase (BACE1). : J Biol Chem. 2003 Apr 25;278(17):14865-71.
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery with styrofoam box with ice packs. International delivery is also available: Please inquire for costs.
