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InformationHUMAN AMYLOID-BETA 1-42N ELISA KIT, 6.25- 400 pg/mL Assay Range, IBL 27712: kw. AB42, beta-amyloid 1-42N, amyloid beta
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Amyloid-Beta 1-42 ELISA Kit (Human Specificity)
Sensitive and selective colorimetric ELISA kit to ABeta (1-42) that uses polyclonal antibodies for the capture and detection antibodies. The antibody recognition domains are positioned at the N and C-terminus. Assay range 6.25-400 pg/ml. Highly selective to amyloid-beta 1-42 with minimal cross reactivity to amyloid-beta 1-40 (see data below).
Inquire for availability
Specifications:
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Complete ELISA kit in sandwich format. Colorimetric detection at 450 nm.
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The ELISA plate is coated with a polyclonal antibody that recognizes the C-terminus of amyloid-beta 1-42. The polyclonal detection antibody recognizes a domain on the N-terminus of Abeta1-42.
- The kit detects amyloid-beta 1-42 in cerebrospinal fluids, cell culture media or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
- Assay range: 6.25 - 400 pg/ml
Protocol Overview
Protocol
Background on Amyloid-Beta (1-42)
Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by certain secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. The prevailing isoforms of Amyloid-Beta are Aβ40 and Aβ42. Aβ40 is the most widespread amyloid-Beta (Aβ) isoform, while Aβ42 is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease.
Methods Overview-
Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Amyloid-beta 1-42N ELISA Kit Validation Data
References
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Selkoe DJ. Normal and abnormal biology of the -Amyloid precursor protein. Annu. Rev. Neurosci. 17: 489-517, 1994.
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Wang R, Sweeney D, Gandy SE, and Sisodia SS. The profile of soluble amyloid protein in cultured cell media. J. Biol. Chem. 271: 31894-31902, 1996.
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Saido T.C., Iwatsubo T., Mann D.M.A., Shimada H., Ihara Y., and Kawashima S. Dominant and differential deposition of distinct -amyloid peptide species, A N3(pE), in senile plaques. Neuron 14, 457-466, 1995.
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Horikoshi Y., Mori T., Maeda M., Kinoshita N., Sato K., Yamaguchi H., A N-terminal-end specific antibody reduced-amyloid in Alzheimer-model mice. Biochem. Biophys. Res. Commun. 325: 384-387, 2004.
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery with styrofoam box and ice packs. International delivery is also available: Please inquire for international delivery costs.
kw. amyloid beta, beta amyloid, elisa, 1-42, abeta, ab
 ELISA Kit-80x80.jpg "Human APLP2 (Amyloid-Like Protein 2; Part APLP2-ELISA")
