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InformationHuman Amyloid-Beta 1-40 (Full length Abeta 1-40) ELISA Kit, Part No. 27714 IBL: kw. AB40N, beta-amyloid 1-40 N, amyloid beta
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AMYLOID-BETA 1-40 (N) ELISA Kit (Human Specificity)
Discontinued. See kit Part No. 27718
Sensitive and selective colorimetric ELISA kit to full length ABeta (1-40) peptide with assay range 15.6-1000 pg/ml. Highly selective to amyloid-beta 1-40 with minimal cross reactivity to amyloid-beta 1-42 (see data below). The affinity purified monoclonal capture antibody reacts with an epitope near residue 40 on the C-terminus whereas the affinity purified polyclonal-HRP detection antibody reacts with the N-terminus region; therefore this ELISA kit provides epitope recognition on opposite ends of the Abeta1-40 peptide.
Discontinued: view related kit 27718 to Abeta 1-40.
A related kit to Abeta 1-42 is also available.
Lead time estimate: 2 weeks
Specifications:
- 15.6 - 1000 pg/ml assay range. 2.77pg/ml sensitivity.
- Complete ELISA kit in sandwich format. Colorimetric detection at 450 nm.
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ELISA plate is coated with an affinity purified capture monoclonal antibody that recognizes the C-terminus of amyloid-beta 1-40 near residue 40. The detection antibody is a polyclonal antibody that recognizes the N-terminal region.
- The kit detects amyloid-beta 1-40 in EDTA-plasma, cerebrospinal fluids, serum, cell culture media or brain tissue extract.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate; overnight. Detection antibody: 1 hr
- Virtually no cross-reactivity with amyloid-beta 1-42.
Protocol Overview
ProtocolEpitope Positions
Background on Amyloid-Beta (1-40)
Amyloid-beta (Abeta) is generated through amyloid precursor protein (APP) cleavage by secretases. Beta-secretase cleaves an extracellular portion of APP and frees a soluble portion. Gamma-secretase cleaves APP within the transmembrane domain, creating amyloid-beta (Aβ) isoforms with lengths between 36-43 amino acid residues. The prevailing isoforms of amyloid-Beta are Aβ40 and Aβ42. Aβ40 is the most widespread amyloid-Beta (Aβ) isoform, while Aβ42 is more fibrillogenic and is the principal Abeta peptide associated with Alzheimer’s disease. The affinity purified monoclonal antibody to the C-terminus percolated on the ELISA plate and the affiniy purified polyclonal HRP conjugated detection antibody that reacts with the N-terminus generates an ELISA kit that is highly selective to Aβ40 with insignificant cross-reactivity to Aβ42.
Methods Overview
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Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Amyloid-beta 1-40N ELISA Kit Validation Data
References
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Selkoe DJ. Normal and abnormal biology of the β-Amyloid precursor protein. Annu. Rev. Neurosci. 17: 489-517, 1994.
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Wang R, Sweeney D, Gandy SE, and Sisodia SS. The profile of soluble amyloid β protein in cultured cell media. J. Biol. Chem. 271: 31894-31902, 1996.
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Saido T.C., Iwatsubo T., Mann D.M.A., Shimada H., Ihara Y., and Kawashima
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S. Dominant and differential deposition of distinct β-amyloid peptide species, AβN3(pE), in senile plaques. Neuron 14, 457-466, 1995.
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Horikoshi Y., Mori T., Maeda M., Kinoshita N., Sato K., Yamaguchi H., Aβ N-terminal-end specific antibody reduced β-amyloid in Alzheimer-model mice. Biochem. Biophys. Res. Commun. 325: 384-387, 2004
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery in styrofoam box with ice packs. International delivery is also available: Please inquire for international delivery costs.
kw. AMYLOID BETA ELISA KIT, ABETA1-42, ABETA42, AB42, BETA AMYLOID 42, 1-42, ELISA KIT
