Human Cyclophilin B ELISA Kit (CYPB, PPIB, Peptidylprolyl Isomerase B) Part No. hCYPB-ELISA

• Fully pre-configured sandwich ELISA providing convenience, high sensitivity and selectivity.
• Affinity purified capture antibody pre-coated on plate.  Separate HRP conjugated detection antibody also provided.
  
• Strip plate configuration that enables separate use and storage of sections of the ELISA plate.
• Rapid assay format.  30min incubation with both capture and detection antibody at 37^oC.
  
• Sensitive picogram colorimetric detection at 450 nm.
• Assay range:  30pg/mL-1ng/mL.

Overview:  Cyclophilin B (CyPB) is a cyclosporine-binding protein located mainly within the endoplasmic reticulum.  Cyclophilin B has a signal sequence and is therefore associated with the secretory pathway and released in biological fluids.   

Given that Cyclophilin B is a soluble protein in the secretory pathway, the primary fractions to detect and assay its presence are in biological fluids, such as serum and plasma, cell culture supernatant, and cell and tissue lysates.   Use the isolation methods suggested in the protocol manual to prepare samples using biological fluids and cell culture supernatant.  To assay in cell or tissue lysates, use a non-denaturing lysis buffer to solubilize membranes and suspend intracellular Cyclophilin B into the lysis buffer supernatant.  The  protocol associated with the FIVEphoton Biochemicals ELISA Lysis and Protein Extraction Buffer (Part No. ELSP-1) provides detailed instructions to isolate intracellular proteins by cell lysis.