Protease Inhibitor Cocktail with EDTA 100X, Water Soluble
(Makes a 1 ml 100x protease inhibitor cocktail solution when suspended in dH20. Provided lyophilized)
-
Broad spectrum and water soluble protease inhibitor cocktail.
-
Protease inhibitor cocktail includes inhibition of serine and cysteine proteases and aminopeptidases.
-
Inhibits calcium dependent proteases.
-
Suspend in dH20. Add aliquot of 100X Protease Inhibitor Cocktail directly to cell lysates.
Directions: Suspend lyophilized material in 1 ml dH20 to make 100X solution. Store aliquots at -20 degrees C.
Protease Inhibitor Cocktail with EDTA Contents (at 100X concentration)
-
AEBSF; 50mM.
-
Aprotinin; 30 uM.
-
E-64; 1 mM.
-
Bestatin; 1mM.
-
Leupeptin; 1mM.
-
EDTA; 5mM.
Specification Sheet
Related Product: Protease Inhibitor Cocktail w/o EDTA, water soluble: click here
Protease Inhibitor References
-
Arai T, Ikarashi Y, Okamoto K, Kuribara H, and Maruyama Y. Memory disturbance and hippocampal degeneration induced by continuous intraventricular infusion of a protease inhibitor, leupeptin. Brain Res 754: 157-162, 1997.
-
Christoffers KH, Khokhar A, Chaturvedi K, and Howells RD. Inhibition of mu and delta opioid receptor ligand binding by the peptide aldehyde protease inhibitor, leupeptin. Regul Pept 105: 9-14, 2002.
-
Citron M, Diehl TS, Capell A, Haass C, Teplow DB, and Selkoe DJ. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron 17: 171-179, 1996.
-
Hussein EM, Dawoud HA, Salem AM, and Atwa MM. Antiparasitic activity of cystine protease inhibitor E-64 against Giardia lamblia excystation in vitro and in vivo. J Egypt Soc Parasitol 39: 111-119, 2009.
-
Jose J and Zangen D. Autodisplay of the protease inhibitor aprotinin in Escherichia coli. Biochem Biophys Res Commun 333: 1218-1226, 2005.
-
Nakabo Y and Pabst MJ. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol 60: 328-336, 1996.
-
Rudzinski T, Mussur M, Gwiazda Z, and Mussur M. Protease inhibitor leupeptin attenuates myocardial stunning in rat heart. Med Sci Monit 10: BR4-10, 2004.
-
Sekiguchi T, Hosoyama Y, and Miyata S. Effects of proteasome inhibitor (lactacystin) and cysteine protease inhibitor (E-64-d) on processes of mitosis in Xenopus embryonic cells. Zoolog Sci 19: 1251-1255, 2002.
-
Tissot G, Canard H, Nadai M, Martone A, Botterman J, and Dubald M. Translocation of aprotinin, a therapeutic protease inhibitor, into the thylakoid lumen of genetically engineered tobacco chloroplasts. Plant Biotechnol J 6: 309-320, 2008.
-
Tsuji S and Matsushita H. Successful treatment of murine muscular dystrophy with the protease inhibitor bestatin. J Neurol Sci 72: 183-194, 1986.
-
Vlasova TI and Vanyushin BF. DNA methylation by wheat cytosine DNA methyltransferase: modulation by protease inhibitor E-64. Biochem Mol Biol Int 45: 145-153, 1998.
Protease Inhibitor Cocktail Inhibitor with EDTA: Storage, Safety, Shipping.
Storage: Lyophiilized material ships at ambient temperature. Store at -20 degrees C upon arrival. -20 degrees C when diluted in dH20.
Safety: Harmful. Avoid ingestion, eye and skin contact. Wear protective clothing, gloves and eye protection.
Shipping options for Protease Inhibitor Cocktail: Two day domestic delivery (Shipping charges: $15.00). Domestic overnight Fedex delivery (shipping charges $27.00). International delivery is also available.
PROTEASE INHIBITOR, COCKTAIL, AEBSF, APROTININ, E-64, LEUPEPTIN, BESTATIN
|