Human Neprilysin (NEP) ELISA Kit ( Part NEP-ELISA Biotin) kw: neurosin
Human Neprilysin (NEP) ELISA Kit
Dynamic Assay Range: 5pg/ml - 1000pg/ml. Sensitivity: 3.8pg/ml.
Lead Time: Approximately 2 weeks
Fully guaranteed to meet specifications
Specifications
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a provided biotin-linked detection antibody and provided streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
Sandwich ELISA Methods to NEP ![]()
ELISA Assay Steps
Gerneral References on NEP
1. Papandreou CN, Nanus DM (January 2010). "Is methylation the key to CD10 loss?". J. Pediatr. Hematol. Oncol. 32 (1): 2–3.
2. Wang DS, Iwata N, Hama E, Saido TC, Dickson DW (October 2003). "Oxidized neprilysin in aging and Alzheimer's disease brains". Biochem. Biophys. Res. Commun.310 (1): 236–41.
3. Oefner C, Roques BP, Fournie-Zaluski MC, Dale GE (February 2004). "Structural analysis of neprilysin with various specific and potent inhibitors". Acta Crystallogr. D Biol. Crystallogr. 60 (Pt 2): 392–6.
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4°C. 6 months.
Shipping. Overnight domestic delivery recommended with ice packs. Also avaialble for international delivery; please inquire for shipping cost.
|