Human Cystatin C ELISA Kit ( Part hCysC-Biotin) kw: cystatin c, neurosin

Human Cystatin C ELISA Kit ( Part hCysC-Biotin) kw:  cystatin c, neurosin
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Price: $655.00
Availability: In Stock
Model: hCysC-Biotin

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Human Cystatin C ELISA Kit

Dynamic Assay Range: 312pg/ml - 20ng/ml.  
Lead Time:  Approximately 1.5 weeks

Specifications
  • Pre-configured ELISA kit in sandwich assay format.
  • Dynamic Assay Range: 312pg/ml - 20ng/ml.  
  • Rapid ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with biotinylated detection antibody and streptavidin-HRP prior to addition to plate.


Number of Assays
Cost
96T
$655
2 x 96T
$1200


Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.

  Protocol


   Sandwich ELISA Methods to Kallikrein-6 (KLK6)                                                       


ELISA Assay Steps
  1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.



Introduction to Cystatin C:   
Cystatin C is a protein encoded by the CST3 gene. It is mainly used as a biomarker of kidney disease but has more recently been associated with its relation to determining new-onset or deteriorating cardiovascular disease, as well as its relation to diseases involving amyloid such as Alzheimer's. It also acts as an inhibitor of cysteine proteases and lysosomal proteinases.  Cystatin C is found in all body fluids and tissues. 

For research only.  Not for diagnostics.
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4°C. 6 months.
Shipping.  Overnight delivery with styrofoam box and ice packs.  Available for international delivery; please inquire for shipping cost. 


 

Category Download Link
Protocol Manual click here
MSDS click here