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InformationHuman Amyloid Precursor Protein (APP) ELISA Kit 96T (Part No. FPBHAPP-1)
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Human Amyloid Precursor Protein (APP) ELISA Kit
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Species
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Human
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Pack Size
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1x96 well plate
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Assay Range
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312pg/ml-20,000pg/ml
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Sensitivity
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< 10 pg/ml
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Cross Reactivity
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There is no detectable cross-reactivity with other relevant proteins.
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Storage
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Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
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Sample Matrix
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This kit is used for quantitative detection of human APP in cell culture supernates.
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View Protocol
Antibody Epitope Positions
Kit Components
96-well plate precoated with anti- human APP antibody 1 ST0000-20 Lyophilized recombinant human APP standard 20ng/tube×2 AR1107 Biotinylated anti- human APP antibody 130ul(dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130ul(dilution 1:100) AR1106-1 Sample diluent buffer 30 ml AR1106-2 Antibody diluent buffer 12ml AR1106-3 ABC diluent buffer 12ml AR1104 TMB color developing agent 10ml AR1105 TMB stop solution 10ml
Background
Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.
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1.PDB 1RW6; Wang Y, Ha Y (August 2004). "The X-ray structure of an antiparallel dimer of the human amyloid precursor protein E2 domain". Mol. Cell 15 (3): 343–53.
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2.Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J (July 2006). "Synapse formation and function is modulated by the amyloid precursor protein". J. Neurosci. 26 (27): 7212–21.
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3.Turner PR, O'Connor K, Tate WP, Abraham WC (May 2003). "Roles of amyloid precursor protein and its fragments in regulating neural activity, plasticity and memory". Prog. Neurobiol. 70 (1): 1–32.
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4.Duce JA et al. (2010). "Iron-Export Ferroxidase Activity of ß- Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease". Cell 142 (6): 857–67.
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