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InformationADAM10 ELISA KIT, 50-1500pg/ml Assay Range, (A Disintegrin And Metalloprotease 10; Part No. ADAM10-ELISA (96T); kits available for human, rabbit, rat and mouse
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ADAM10 ELISA Kits (96T)
Email customersupport@fivephoton.com for availability of this kit
Part No. ADAM10-ELISA (96T)
(A Disintegrin And Metalloprotease 10; Separate kits available for human, mouse, rat and rabbit)
Select the species specific kit in the available options menu.
Lead time: Approximately 3 weeks
Fully guaranteed to meet specifications.
Highlights
 View protocol
ADAM10 ELISA Kit Availability And Specifications
Use Available Options menu to select the species specificity of the ADAM10 ELISA Kits.
Background
Members of the ADAM family are transmembrane proteins with a unique structure possessing both potential adhesion and protease domains. Sheddase, a generic name for the ADAM metallopeptidase, functions primarily to cleave membrane proteins at the cellular surface. Once cleaved, the sheddases release soluble ectodomains with an altered location and function.
Although a single sheddase may “shed” a variety of substances, multiple sheddases can cleave the same substrate resulting in different consequences. In neurons, ADAM10 is the most important enzyme with α-secretase activity for proteolytic processing of the amyloid precursor protein.
Methods Overview: Sandwich ELISA Format
The included ELISA 96-well micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and then color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is added to block further reaction between HRP and the colorimetric substrates, converting the solution into a yellow coloration. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm. This assay requires approximately 1.5 hrs to complete at 37oC, which includes incubation with antibodies, washes and color reaction.
This ELISA assay kit provides a standard peptide solution with known concentration to calibrate absorbance readings to concentration.
Representative Sandwich ELISA Methods to ADAM10 
OVERVIEW OFrREPRESENTATIVE ELISA METHOD
1. Equilibrate plate to ambient temperature
2. Add sample, incubate 30 min - 1hr, wash
3. Add HRP-Antibody, incubate 30 min - 1 hr, wash
4. Add the color reagent, incubate 15 min
5. Add the stop solution
Protocol
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4oC, 6 months. Strip plate sections, standard and other solutions can also be stored at -20oC longer term. Store opened ELISA plate in sealed zip lock bag.
Shipping. Overnight domestic delivery recommended with styrofoam box/ice pack. Also available for international delivery; please inquire for shipping cost.
Keywords
Human ADAM10 ELISA Kit: Part No. hADAM10-ELISA (96T)
Mouse ADAM10 ELISA Kit: Part No. mADAM10-ELISA (96T)
Rat ADAM10 ELISA Kit: Part No. rADAM10-ELISA (96T)
Rabbit ADAM10 ELISA Kit: Part No. rbADAM10-ELISA (96T)
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