Extracellular Immunofluorescence Labeling (Staining) Kit (EIF-1)

Extracellular Immunofluorescence Labeling (Staining) Kit (EIF-1)
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Price: $350.00
Availability: In Stock
Model: EIF-1
Manufacturer: Fivephoton Biochemicals

Extracellular Immunofluorescence Cell Labeling Kit
  • Selective immunofluorescence labeling of extracellular epitopes.
  • Membranes are not permeabilized during immunofluorescence labeling.
  • Ideal for protein trafficking and receptor-cell signaling studies.
  • Suitable for mapping extracellular loops and protein topology.
  • Angled sharp forceps are included to handle glass cover slips.
For 40 microscope slides. 


Extracellular Immunofluorescence Cell Labeling Kit

Labeling of extracellular epitopes for immunofluorescent detection is an important method to characterize protein trafficking in cells as well as in mapping of extracellular domains of multi-pass transmembrane proteins.  Extracellular immunofluorescence labeling procedures are employed to determine whether transmembrane proteins are positioned on the cell surface.  This extracellular immunofluorescence staining (labeling) Kit  (Part No. EIF-1) does not permeabilize cell membranes, enabling selective immunofluorescent  labeling of extracellular epitopes.  Immunofluorescence is displayed on the cell periphery after employing the reagents and protocol of this kit.  Note that selective extracellular immunofluorescence labeling requires the use of antibodies that exclusively recognize extracellular epitopes.

In contrast to the Extracellular Immunofluorescence Labeling Kit, the FIVEphoton Biochemicals Intracellular Immunofluorescence Staining (labeling) Kit (INF-1;link) provides reagents that permeabilize membranes, resulting in the detection of immunofluorescence associated with both intracellular and extracellular epitopes.  A permeabilization buffer is included with the Intracellular Immunofluorescence Labeling Kit that enables detection of antigens in virtually all cellular compartments.


Extracellular Immunofluorescence Kit Contents

Item No. Description Volume
PBS-1 PBS 10X, pH 7.4 32 ml
Q-1 Quencing Buffer 10X 26 ml
EB-1
Extracellular Blocking Buffer 10X
10 ml
E-1
Extracellular Detection Buffer 10X
80 ml
SAF-3 Forceps Set NA

Safety:  Contains irritants.  Avoid ingestion, skin and eye contact.
Storage:  -20oC
Shipping Options:   Ships at ambient temperature:  On arrival, store at -20oC.  Overnight domestic and international priority shipping available.



 

Category Download Link
Protocol Manual click here
MSDS click here



 

Extracellular Immunofluorescence Labeling (Staining) Kit (EIF-1)
Click to enlarge