GLP-1 ACTIVE (Glucagon Like Peptide-1) ELISA KIT (Human, Mouse, Rat Reactivity, 96T: Part 27788)

GLP-1 ACTIVE (Glucagon Like Peptide-1) ELISA KIT (Human, Mouse, Rat Reactivity, 96T: Part 27788)
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Price: $865.00
Availability: In Stock
Model: 27700 IBL
Manufacturer: IBL Japan

GLP-1 Active ELISA Kit

Part No. 27788
Conjugate HRP
Species Human, Mouse, Rat
Sample Types EDTA-plasma, cell culture supernatant
Assay Range 0.25 ~ 16 pmol/L
Primary Reaction 2 ~ 8°C Overnight
Secondary Reaction 60 minutes at 2 ~8°C
Sensitivity 0.09 pmol/L
Specificity GLP-1 (7-36) amide 100%
GLP-1 (7-37)100%
GLP-1(1-37)0.04%
GLP-1(9-36) amide 0.02%
Glucagon N.D.
Human GLP-2 N.D.
Human GIP(1-42) N.D.
Human GIP(3-42) <0.01%
Mouse GIP(1-42) N.D.
Rat GIP(1-42) N.D.
Storage  2 - 8 ℃
Package Size 1 96 Well



GLP-1 Background

Glucagon-like peptide-1 (GLP-1) is a 30 amino acid long peptide deriving from the tissue-specific posttranslational processing of proglucagon.  The initial product GLP-1 (1–37) is susceptible to amidation and proteolytic cleavage which gives rise to the two truncated and equipotent biologically active forms, GLP-1 (7–36) amide and GLP-1 (7–37). Active GLP-1 composes two alpha-helices from amino acid position 13–20 and 24–35 separated by a linker region.

Alongside glucose-dependent insulinotropic peptide (GIP), GLP-1 is an incretin; thus, it has the ability to decrease blood sugar in a glucose-dependent manner by enhancing the insulin secretion. Beside the insulinotropic effects, GLP-1 has been associated with numerous regulatory and protective effects. The action of GLP-1 is preserved in patients with type 2 diabetes and substantial pharmaceutical research has therefore been directed towards the development of GLP-1-based treatment.


         Methods Overview

  1. Equilibrate plate to RT
  2. Add sample to plate, incubate overnight
  3. Add detection antibody, incubate 1 hr
  4. Add color reagents, 30 min
  5. Add stop solution.  Measure OD at 450nm

              


  Protocol Overview

  Protocol


Data
 

Storage:  4oC, 1 year
Safety:  Stop solution contains acid. Avoid contact, wear eye protection.
Shipping:  Overnight delivery with styrofoam box and ice packs.  International delivery is also available:  Please inquire for costs.


 

 

Category Download Link
Protocol Manual click here
MSDS click here