QA, Quinolinic Acid ELISA Kit (0.187ng/ml Sensitivity, 0.2-60ng/ml Assay Rang; Part No hQA-ELISA1; 96T)

• Direct sandwich ELISA format providing convenience and high sensitivity.
• Assay ranges 0.2 ng/ml to 60 ng/ml.
• Sensitivity:  0.187 ng/ml 
• Strip plate format allowing separate use and storage of ELISA plate sections.
• Rapid protocol:  Only one incubation.  Total protocol time approximately 1.5 hrs.
• Colormetric detection at 450nm.

Protocol

 

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1. 8 x 12 well removable section strip plate coated with anti-QA antibody
2. Anti-QA polyclonal detection antibody conjugated with biotin
3. Steptavidin-HRP
4. Protein Standard
5. Standard diluent
6. Concentrated wash solution
7. Chromogen solutions A and B
8. Stop solution
9. Plate sealers

Quinolinic Acid (QA) is a very neurotoxic chemical associated with many brain disorders, including neurodegeneration (alzheimers, ischemia, and amyotrophic lateral sclerosis [ALS]) and psychiatric illnesse like bipolar disorder, depression, and schizophrenia.  Quinolinic acid is also correlated with acquired dementia in some AIDS and HIV patients due to the activity of macrophages in the brain.  QA is also the biosynthetic precursor to nicotine.  

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Methods Overview:

The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.  

   Sandwich ELISA Methods                                                                                                  

ELISA Assay Steps

1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37^oC.  Wash 5 times.
3. Apply chromogens, incubate, then stop reaction.

 

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References

1. Chaiyasut, C.; Sivamaruthi, B.S.; Kesika, P.; Khongtan, S.; Khampithum, N.; Thangaleela, S.; Peerajan, S.; Bumrungpert, A.; Chaiyasut, K.; Sirilun, S.; Sittiprapaporn, P. Synbiotic Supplementation Improves Obesity Index and Metabolic Biomarkers in Thai Obese Adults: A Randomized Clinical Trial. Foods 2021, 10, 1580. https://doi.org/10.3390/foods10071580