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Tyrosine Hydroxylase ELISA Kit (TH, Part TH-ELISA)

Tyrosine Hydroxylase ELISA Kit (TH, Part TH-ELISA)
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Price: $655.00
Availability: In Stock
Model: TH-ELISA

Available Options:
Format 1:
DetailsManuals & Downloads
Tyrosine Hydroxylase ELISA Kits 
 
Highly sensitive sandwich ELISA Kits with pg/ml detection and rapid protocol for rat, haman, mouse or porcine tyrosine hydroxylase.  96T

Fully guaranteed to meet specifications
 
Tyrosine Hydroxylase ELISA Kits.  Separate kits are available for human, mouse, rat, and porcine.
  • Fully pre-configured sandwich ELISA format providing optimal convenience, specificity and sensitivity
  • Strip plate configuration allowing separate use and storage of 96-well ELISA plate sections
  • Affinity purified polyclonal or monoclonal capture antibodies coated on plate, depending on kit format.  Separate HRP-conjugated detection antibody
  • Rapid colorimetric detection. 1 hr total incubation at 37oC
  • Suitable for CSF, cell and tissue lysates and homogenates, other biological fluids
  • Highly sensitive: measures Tyrosine Hydroxylase concentration in pg/ml
  • Available for Human, mouse, rat, and porcine Tyrosine Hydroxylase

Specifications of Tyrosine Hydroxylase ELISA Kits

Format 1.  View this product at these links:  

 Species
 Standard 
 Assay Range
 Sensitivity
 Price
 Human 192 ng/ml 0.05 ng/ml -  20 ng/ml
 26 pg/ml
 $655
 Mouse 80 ng/ml 0.5 ng/ml -200 ng/ml 270 pg/ml
 $655
 Rat 180 ng/ml 0.5 ng/ml - 160 ng/ml
 230 pg/ml
 $655
 Porcine 320 ng/ml 1 ng/ml - 300 ng/ml 230pg/ml
 $655
 
Use Available Options menu to select species.

Background:  Tyrosine Hydroxylase catalyzes the first step in catecholamine production by converting the amino acid L-tyrosine to the catecholamine precursor L-3,4-dihydroxyphenylalanine (L-DOPA) using Oxygen, Fe3+, and tetrahydrobiopterin as cofactors.  This first step is the rate limiting step in the catecholamine synthesis pathway. 

In humans, Tyrosine Hydroxylase is found in neurons of the central and peripheral nervous systems and in the cells of the adrenal medulla. 

Methods Overview 

Sandwich ELISA Format 1:  The ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with provided  biotin-linked detection antibody and streptavidin-HRP solutions, which are then added to the plate and incubated for 60min.  The plate is washed  and color reagents are added, resulting in a reaction with HRP.  A stop solution is applied and the resulting yellow solution is measured at 450nm.
 
*(Use the protocol shipped with the kit for your experiment).
   Representative Sandwich ELISA Methods                                                                                    
Steps of Sandwich ELISA Format 1
  1. Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.
 

References on Tyrosine Hydroxylase
  1. Nagatsu, T., Levitt, M., & Udenfriend, S. (1964). Tyrosine hydroxylase the initial step in norepinephrine biosynthesis. Journal of Biological Chemistry, 239(9), 2910-2917.
  2. Spector, S., Sjoerdsma, A., & Udenfriend, S. (1965). Blockade of endogenous norepinephrine synthesis by α-methyl-tyrosine, an inhibitor of tyrosine hydroxylase. Journal of Pharmacology and Experimental Therapeutics, 147(1), 86-95.
  3. Goodwill, K. E., Sabatier, C., Marks, C., Raag, R., Fitzpatrick, P. F., & Stevens, R. C. (1997). Crystal structure of tyrosine hydroxylase at 2.3 Å and its implications for inherited neurodegenerative diseases. Nature Structural & Molecular Biology, 4(7), 578-585.
  4. Udenfriend, Sidney. "B. Tyrosine hydroxylase." Pharmacological reviews 18.1 (1966): 43-51.
For research only.  Not for diagnostics of disease in humans or animals.
Lead  time:  approximately 2 weeks.
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4oC, 6 months.  Longer term storage of sections of the strip plate and standards at -20oC.  Seal and store 96-well ELISA plate in a zip locks bag after opening.
Shipping.  Overnight domestic delivery recommended (Flat rate Fedex cost for continental US: $45).  Also available for international delivery; please inquire for shipping cost. 

KW:  Tyrosine Hydroxylase ELISA Kit
Human Tyrosine Hydroxylase ELISA Kit
Mouse Tyrosine Hydroxylase ELISA Kit
Rat Tyrosine Hydroxylase ELISA Kit
Porcine Tyrosine Hydroxylase ELISA Kit

 
 


 

Category Download Link
Protocol Manual Format I
 click here
Protocol Manual Format II click here
MSDS click here