Categories
 
Information
 

RAT sAPPα 150pg/ml-30ng/ml ELISA KIT (soluble amyloid precursor protien alpha, sAPPalpha, sAPPa, sAPP alpha, Part No. rsAPPalpha-ELISA2)

RAT sAPPα 150pg/ml-30ng/ml ELISA KIT (soluble amyloid precursor protien alpha, sAPPalpha, sAPPa, sAPP alpha, Part No. rsAPPalpha-ELISA2)
Click to enlarge
Price: $645.00
Availability: In Stock
Model: rsAPPalpha-ELISA2

DetailsManuals & DownloadsRelated Products

Rat Soluble Amyloid Precursor Protein Beta ELISA Kit (sAPPα, sAPPalpha) 96T

Dynamic Assay Range: 150pg/ml - 30ng/ml.  Sensitivity: 0.122pg/ml.
Lead Time:  Approximately 1.5 weeks

Specifications
  • Fully pre-configured ELISA kit in sandwich assay format.
  • Dynamic Assay Range: 150pg/ml - 30ng/ml.  Sensitivity: 0.122pg/ml.
  • Rapid ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate.
  • 96T format.

Number of Assays
 Cost
96T
 $645
Background
sAPPα is the extracellular soluble fragment of amyloid precursor protein cleaved by "alpha-secretase."  Generation of  sAPPα by "alpha-secretase" prevents development of fibrillogenic amyloid-beta since the cleavage site is within the amyloid-beta molecule.  The generation of sAPPalpha may therefore be neuroprotective.  sAPPα may function in neuronal excitability, synaptic plasticity, neurite outgrowth, synaptogenesis, and cell survival.  sAPPalpha also appears to modulate potassium channels, N-methyl-D-aspartate receptors, and the transcription factor NF kappa B1.

1.  Mttson MP.  Physiol Rev. 1997 Oct;77(4):1081-132.  Cellular actions of beta-amyloid precursor protein and its soluble and fibrillogenic derivatives.
Methods Overview:
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
   Sandwich ELISA Methods to sAPPbeta                                                      

ELISA Assay Steps
  1. Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.


For research only.  Not for diagnostics.
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4°C. 6 months.
Shipping.  Overnight domestic delivery recommended with ice pack (cost: $56 for 1-2 kits).  Also avaialble for international delivery; please inquire for shipping cost. 
kw: sAPPa, sAPPalpha, sAPP alpha, soluble amyloid precursor protein alpha ELISA kit


 

Category Download Link
Protocol Manual click here
MSDS click here



 

HUMAN AMYLOID-BETA (1-42) ELISA KIT - 2.5pg/ml SENSITIVITY: Synonyms: Beta-Amyloid 1-42, AB(1-42), ABeta(1-42. Part No. hAbeta42 ELISA (kw. amyloid beta, beta amyloid)
HUMAN AMYLOID-BETA (1-42) ELISA KIT - 2.5pg/ml SENSITIVITY: Synonyms: Beta-Amyloid 1-42, AB(1-42), ABeta(1-42. Part No. hAbeta42 ELISA (kw. amyloid beta, beta amyloid)
hAbeta42 ELISA
$655.00
HUMAN AMYLOID-BETA 1-38 (FL) ELISA KIT (27717; kw. AB38, beta-amyloid 1-38, amyloid beta)
HUMAN AMYLOID-BETA 1-38 (FL) ELISA KIT (27717; kw. AB38, beta-amyloid 1-38, amyloid beta)
27717
$840.00
Human BACE1 ELISA Kit (Human Beta-site APP Cleaving Enzyme 1, Part No. 27752 IBL: kw. BACE1
Human BACE1 ELISA Kit (Human Beta-site APP Cleaving Enzyme 1, Part No. 27752 IBL: kw. BACE1
27752
$660.00
Human Amyloid Precursor Protein (APP) ELISA Kit 96T (Part No. FPBHAPP-1)
Human Amyloid Precursor Protein (APP) ELISA Kit 96T (Part No. FPBHAPP-1)
FPBHAPP-1(96T)
$685.00
HUMAN ALPHA SYNUCLEIN SNCA ELISA KIT (α-synuclein, alpha-synuclein, alpha-synuclein, 27740)
HUMAN ALPHA SYNUCLEIN SNCA ELISA KIT (α-synuclein, alpha-synuclein, alpha-synuclein, 27740)
27740
$895.00