Categories
 
Information
 

Rat Kallikrein-6 (KLK6) ELISA Kit ( Part rKLK6-Biotin) kw: kallikrein 6, neurosin

Rat Kallikrein-6 (KLK6) ELISA Kit ( Part rKLK6-Biotin) kw: kallikrein 6, neurosin
Click to enlarge
Price: $635.00
Availability: In Stock
Model: rKLK6-Biotin

Available Options:
DetailsManuals & DownloadsRelated Products

Rat Kallikrein-6 (KLK6) ELISA Kit

 
Dynamic Assay Range: 200pg/ml - 40ng/ml.  Sensitivity: 0.145ng/ml.
Lead Time:  Approximately 1.5 week

Specifications

  • Fully pre-configured ELISA kit in sandwich assay format.
  • Standard peptide concentration:  48ng/ml
  • Dynamic Assay Range: 200pg/ml - 40ng/ml.  Sensitivity: 0.145ng/ml.
  • Rapid ELISA Protocol:  Requires one 60 min incubation period at 37oC.
  • Samples are mixed with the provided biotinylated detection antibody and provided streptavidin-HRP prior to addition to plate.
  • Available in 48T and 96T formats.
  • 48T Kit Cost:  $335.  96T Kit Cost:  $635. 2 x 96T Cost:  $1150
 
Number of Assays
Cost
48T
$355
96T
$635
2 x 96T
$1150
  Protocol

Methods Overview:

The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  A 60 minute incubation period at 37oC is used, and then the plate is subsequently washed.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the now yellow solution is measured at 450nm.  

   Sandwich ELISA Methods to Kallikrein-6 (KLK6)                                                       

ELISA Assay Steps
  1. Mix sample, provided biotinylated-detection antibody and provided streptavidin-HRP.
  2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37oC.  Wash 5 times.
  3. Apply chromogens, incubate, then stop reaction.


Introduction to Kallikrein-6:   
Kallikrein-6 is a protein with serine protease activity that is detected with grey matter neurons and colocalizes with pathological inclusions. Kallikreins have many known functions; they are associated with carcinogenisis and are thought to be disease biomarkers.  Kallikrein-6 has been found to cleave amyloid precursor protein as well as myelin basic protein, gelatin, casein, and many extracellular matrix proteins.  Kallikrein-6 also cleaves alpha-synuclein, preventing polymerization. 
Gerneral References on Kallikrein-6
  1. Ashby EL, Kehoe PG, Love S. Kallikrein-related peptidase 6 in Alzheimer’s disease and vascular dementia. Brain Research 1363: 1-10, 2010.
  2. Diamandis EP, Yousef GM, Luo LY, Magklara A, Obiezu CV. The new human kallikrein gene family: implications in carcinogenesis. Trends in endocrinology and metabolism TEM 11: 54-60, 2000.
  3. Magklara A, Mellati AA, Wasney GA, Little SP, Sotiropoulou G, Becker GW, Diamandis EP. Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors. Biochemical and Biophysical Research Communications307: 948-955, 2003.
  4. Menendez-Gonzalez M, Castro-Santos P, Suarez A, Calatayud MT, Perez-Pinera P, Martinez M, Ribacoba R, Gutierrez C. Value of measuring plasmatic levels of neurosin in the diagnosis of Alzheimer’s disease. Journal of Alzheimers disease JAD 14: 59-67, 2008.
  5. Ogawa K, Yamada T, Tsujioka Y, Taguchi J, Takahashi M, Tsuboi Y, Fujino Y, Nakajima M, Yamamoto T, Akatsu H, Mitsui S, Yamaguchi N. Localization of a novel type trypsin-like serine protease, neurosin, in brain tissues of Alzheimer’s disease and Parkinson's disease.Psychiatry and Clinical Neurosciences 54: 419-426, 2000.
  6. Okui A, Kominami K, Uemura H, Mitsui S, Yamaguchi N. Characterization of a brain-related serine protease, neurosin (human kaillikrein 6), in human cerebrospinal fluid. NeuroReport 12: 1345-1350, 2001.
  7. Shan SJC, Scorilas A, Katsaros D, Diamandis EP. Transcriptional upregulation of human tissue kallikrein 6 in ovarian cancer: clinical and mechanistic aspects. British Journal of Cancer 96: 362-372, 2007.
For research only.  Not for diagnostics.
Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.
Storage:  4°C. 6 months.
Shipping.  Overnight domestic delivery recommended with ice pack (cost: $42 for 1-2 kits).  Also avaialble for international delivery; please inquire for shipping cost. 


 

Category Download Link
Protocol Manual click here
MSDS click here

 



 

Fluorescent Dopamine | Fluorescent Neurotransmitter - Dansyl 5-C Conjugated Dopamine | FIVEphoton Biochemicals | Dansyl-Dopamine | Part DnsylD-1
Fluorescent Dopamine | Fluorescent Neurotransmitter - Dansyl 5-C Conjugated Dopamine | FIVEphoton Biochemicals | Dansyl-Dopamine | Part DnsylD-1
DnsylD-1
$308.00
FIVEPHOTON MEMBRANE POTENTIAL ION CHANNEL ASSAY KIT | Fluorescent Voltage Sensitive Dye Kit (kw. Voltage-sensitive probes: Part MPF-Kit)
FIVEPHOTON MEMBRANE POTENTIAL ION CHANNEL ASSAY KIT | Fluorescent Voltage Sensitive Dye Kit (kw. Voltage-sensitive probes: Part MPF-Kit)
MPF-Kit
$390.00