HUMAN AMYLOID-BETA 1-40 100pg-3000ng/ml Assay Range ELISA KIT (Beta-Amyloid, AB, ABeta). Part No. hAB1-40ELISA (kw. beta amyloid, amyloid beta)

• 100pg - 3000ng/ml assay range: Highly selectivite, sensitive fully pre-configured sandwich ELISA kit
• Strip plate format, allowing for separate section storage
• Picogram detection;  colorimetric read out 
  
• Rapid Assay:  2 hrs incubation at RT or 1 hr at 37^oC
  
• Affinity purified polyclonal capture antibody coated on plate, and separate detection antibody conjugated with HRP
  
• Suitable for CSF, ISF, plasma, cell and tissue homogenates and lysates
• Separate Abeta 1-40 ELISA kits are also availabe for rat, mouse and canine
  

Description	Detection Range	Stnd Peptide
Human Amyloid Beta 1-40 ELISA Kit	100pg - 3000ng/ml	3600ng/ml

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  Representative Amyloid-Beta Sandwich ELISA Method  

Steps of Sandwich ELISA
1.  Equilibrate plate to ambient temperature

2.  Add sample, incubate 30 min - 1hr, wash 

3.  Add HRP-Antibody, incubate 30 min - 1 hr, wash

4.  Add the color reagent, incubate 15 min

5.  Add stop solution

6.  Colorimetric detection, 450 nm

 

 

Amyloid-beta (Abeta) is generated by sequential cleavage of the amyloid precursor protein (APP) by beta and gamma secretases.  Beta-secretase cleaves an extracellular fragment of APP and releases a soluble fragment, whereas gamma secretase cleaves within the transmembrane domain and generates amyloid-beta (Aβ) isoforms of 36-43 amino acid residues in length.  The dominant isoforms of Amyloid-Beta are Aβ₄₀ and Aβ₄₂.   Aβ₄₀ is the most common amyloid-Beta (Aβ) isoform, whereas Aβ₄₂ is the more fibrillogenic and the predominant Abeta peptide associated with Alzheimer’s disease.  Antibodies employed in this amyloid-beta ELISA kit are selective to the Aβ₄₂ isoform of amyloid-beta, with insignificant cross-reactivity to the Aβ₄₀ isoform.

The included ELISA micro-strip plate is provided pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen.  Upon addition of samples to the wells, the antigen in solution binds to the antibody and becomes indirectly linked to the solid support.  After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period.  Unbound antibody is washed, and subsequently color reagents are added, which upon conversion by HRP, become blue colored.  A stop solution is added to block further reaction between HRP and the colorimetric substrates, causing the solution to become yellow.  An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm.  

These ELISA kits provide a standard peptide solution with known concentration to calibrate absorbance readings to concentration.

Safety, Storage, Shipping, Amyloid-Beta ELISA Kits

Safety:  Stop solution contains acid.   Avoid ingestion, skin and eye contact.

Storage:  4^oC, 6 months.  Strip plate sections, standard, and other solutions can also be stored at -20^oC longer term.

Shipping.  Overnight domestic delivery with ice pack/insulated box recommended (cost $52).  Also available for international delivery;  please inquire for shipping cost.

 

kw.  AMYOID BETA, BETA AMYLOID, ABETA, ABETA, ALZHEIMER'S, ELISA, SANDWICH KIT,