Fluorescent Dopamine | Fluorescent Neurotransmitter - Dansyl 5-C Conjugated Dopamine | FIVEphoton Biochemicals | Dansyl-Dopamine | Part DnsylD-1

1. Pharmalogical properties, including affinity, virtually identical to unconjugated dopamine.
   
2. DnsylD-1 operates selectively in uptake and binding assays at nanomolar concentrations. 
3. The native dopamine chemical structure is the major component of this fluorescent catecholamine, generating a fluorescent neurotransmitter molecule with high affinity toward all dopamine receptor subtypes as well as to the dopamine transporter.
4. The small dansyl tag with a 5-carbon polylinker to dopamine generates a stable, bright fluorescent signal with negligible steric hindrance. 
5. The dansyl fluorescent tag offers a large stokes shift that minimizes autofluorescence and non-specific background signals:  (Ex/Em:  313/515nm).
6. The fluorescent signal of DnsylD-1^TM is blockable by standard inhibitors to dopamine receptors and DAT.
7. Standard inhibitors can be employed to distinguish the interaction of DnsylD-1^TM with dopamine receptors and DAT.
8. Fluorescence from DnsylD-1^TM colocalizes with tyrosine hydroxylase and identifies dopaminergic neurons.
9. To assay for dopamine receptor binding, first suspend the provided solid in DMSO (10 - 50 µl).  Centrifuge to clarify, and collect the DMSO soluble fraction, which can be aliquoted and stored at -20^oC:  DnsylD-1^TM when suspended in DMSO and frozen at -20^oC is stable for at least 2 years.  Dilute the DMSO solubilized material into your assay buffer (for example PBS-BSA) to the desired assay concentration.  Detect DnsylD-1^TM by fluorescent microscopy, with a fluorescent plate reader or by flow cytometry.  Set the fluorescent detection device to Ex/Em:  333/515 nm.

• Spectral characteristics and assay wavelengths:  Ex:Em:  333:515 nm
• MW:  499.62 (Mass and QC verified by MALDI)
• Appearance:  lyophilized off-white solid
• Purity:  ≥ 95% (HPLC; view COA for lot purity)
• Solubility:  DMSO
• Analytical QC Test:  MALDI MS and HPLC
• Use at 10X Kd of unlabeled dopamine toward respective dopamine receptor subtype or transporter
• Storage (lyophilized powder):  4^oC, dark, desiccate.  Storage when solubilized in DMSO:  -20^oC, aliquot.
• Shipping:  Ambient temperature.

  Specification Sheet

  COA Current Lots

 

 

Specification Sheet

1. Pharmacological applications as a substitute for radioactive ligand.
2. Dopaminergic neuron detection:  Biomarker detection.
3. Dopamine uptake analysis.
4. Histochemical imaging and quantitation of dopamine receptor expression in vivo and vitro.
5. Dopamine metabolism and turnover analysis.
6. Analysis of disease conditions associated with altered dopamine synthesis, dopamine receptor expression or dopamine uptake:  human disease research applicability in the following:  a)  Parkinson's disease.  b)  Psychological disorder, including ADHD, schizophrenia and substance abuse.  c)  Physiological regulation of cardiovascular, renal and immune function.

Images:  DnsylD-1^TM (Dansyl-Dopamine) bound to neuroblastoma cells and observed by low magnification fluorescent microscopy over a well of a 96-well plate.  Multiple cells are displayed.