MOUSE sAPPBETA-WT ELISA KIT, (2.87pg/ml sensitivity, Soluble Amyloid Precursor Protein Beta ELISA, sappb; IBL 27416)
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Mouse Soluble Amyloid Precursor Protein Beta (sAPPβ) ELISA Kit
Sensitive and selective colorimetric ELISA kit to sAPPβ with assay range 4.1 - 260 pg/ml. Highly selective to sAPPβ with minimal cross reactivity to sAPPα. 96T
Lead time estimate: 2 weeks
Specifications:
Specifications:
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Complete 96T ELISA kit in sandwich format. Colorimetric detection at 450 nm.
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ELISA plate is coated with an affinity purified polyclonal capture antibody that recognizes mouse sAPPβ. The detection antibody is also a polyclonal antibody that recognizes mouse sAPPβ at its opposite terminus.
- The kit has been validated to detect sAPPβ in EDTA-plasma and cell culture media.
- EIA buffer contains 1% BSA, 0.05% Tween 20 in PBS
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Incubation times: Samples with plate: overnight. Detection antibody: 1 hr
- Assay range: 13 pg/ml - 800 pg/ml
- Sensitivity: 2.87 pg/ml
Protocol Overview
Protocol
Data
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Background on sAPPbeta
sAPPbeta is the extracellular soluble fragment of amyloid precursor protein (APP) cleaved by beta-secretase into a 654 amino acid peptide. Cleavage of APP by beta-secretase in the extracellular domain is the first cleavage processing step generating amyloid-beta peptide. Membrane embedded gama-secretase subsequently cleaves APP within the transmembrane domain and generates amyloid-beta peptide of variable lengths.1 This sAPP beta ELISA detects the sAPP beta peptide in cell culture medium and plasma.
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Methods Overview
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Equilibrate plate to RT
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Add sample to plate, incubate overnight
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Add detection antibody, incubate 1 hr
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Add color reagents, 30 min
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Add stop solution. Measure OD at 450nm
Storage: 4oC, 1 year
Safety: Stop solution contains acid. Avoid contact, wear eye protection.
Shipping: Overnight delivery in styrofoam box with ice packs. International delivery is also available: Please inquire for international delivery costs.
kw. SAPPBETA, AMYLOID PRECURSOR PROTEIN BETA, ALZHEIMER'S, ELISA KIT SAPPB,