CRP, C-REACTIVE PROTEIN ELISA KIT (1GNH 0.003 - 0.15 mg/L Sensitivity, 0.005 - 40 mg/L Assay Range: Part CRP-ELISA, m r or c)

• Assay ranges 0.05 mg/L to 40 mg/L depending on species.
• 0.003 mg/L - 0.15 mg/L sensitivity depending on species
• Separate kits are available for mouse, rat and canine CRP. 
• Sandwich ELISA format providing convenience and high sensitivity. 
• Strip plate format allowing separate use and storage of sections of the ELISA plate.
• Rapid protocol:  Only one incubation with sample to antibodies.  Total protocol time approximately 1.5 hrs.
• Colormetric detection at 450nm using a standard absorbance plate reader.

Protocol

 

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1. 8 x 12 well removable section strip plate coated with anti-CRP antibody
2. Anti-CRP polyclonal detection antibody conjugated with biotin
3. Steptavidin-HRP
4. Protein Standard
5. Standard diluent
6. Concentrated wash solution
7. Chromogen solutions A and B
8. Stop solution
9. Plate sealers

C-Reactive Protein (CRP) is a blood plasma protein involved in the inflammatory response.  It activates the complement system through C1q by binding to the surface of dead or dying cells.  C-Reactive Protein is synthesized in response to IL-6 secreted by macrophages and an elevated level of CRP is a marker of inflammation.    It is also correlated with cancer where its role is ambiguous, and unusually high or low levels could have a role in different types of Alzheimer's disease as well.  

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Methods Overview:

The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.  

   Sandwich ELISA Methods                                                                                                  

ELISA Assay Steps

1. Mix sample, biotinylated-detection antibody and streptavidin-HRP.
2. Apply sample and antibody-HRP complex to ELISA plate for 60min at 37^oC.  Wash 5 times.
3. Apply chromogens, incubate, then stop reaction.