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InformationRat Complement Factor D (CFD; Part rCFD-ELISA (96T))
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Rat Complement Factor D (CFD) ELISA Kit
Email customersupport@fivephoton.com for current lead time estimate.
Protocol
Methods Overview
The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen. Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate. The plate is washed after a 60 min incubation period. Color reagents are added, resulting in a reaction with HRP that yields a blue colored solution. A stop solution is applied to terminate the reaction, and the solution is measured at 450nm.
Sandwich ELISA Methods
ELISA Assay Steps
Introduction to Complement Factor D
The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology.
For research only. Not for diagnostics.
Safety: Stop solution contains acid. Avoid ingestion, skin and eye contact.
Storage: 4°C. 6 months.
Shipping. Overnight domestic delivery recommended with ice pack (cost: $42 for 1-2 kits). Also avaialble for international delivery; please inquire for shipping cost
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